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首页> 外文期刊>Life sciences >Glycogen turnover in skeletal muscle is stimulated along with glucose uptake in vivo during contraction.
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Glycogen turnover in skeletal muscle is stimulated along with glucose uptake in vivo during contraction.

机译：在收缩过程中，骨骼肌中的糖原更新以及体内葡萄糖的摄取受到刺激。

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We have shown previously that glycogen synthesis in the heart can be stimulated in vivo by epinephrine. Our aim in this study was to determine whether glycogen synthesis in skeletal muscle can be similarly affected during increased energy expenditure. Left sciatic nerves of anesthetized fasted rats were electrically stimulated to allow left hindlimb muscles to contract for 5, 10, and 20 min. Glycogen contents in the contracting muscles at the end of electrical stimulation were found to be approximately 40% less than resting muscles in the right hindlimbs in all three groups of rats. Accompanying the enhanced glycogenolysis was increased incorporation of the intravenously infused [3-(3)H]-glucose into glycogen. The rate of tritium incorporation into glycogen in the contracting muscle was found to be 34-fold greater than resting muscles. Glucose utilization was determined by the phosphorylation of the intravenously injected [14C]-2-deoxyglucose in the skeletal muscle. The rate of accumulation of [14C]-2-deoxyglucose-6-phosphate in the contracting muscles was found to be 28-fold greater than resting muscles. Glycogen synthesis and glucose uptake indexes, calculated by dividing the radioactivity in [3H]-glycogen and [14C]-2-DGP by the mean specific activity of their respective precursors in the plasma, were not found to be significantly different in the contracting muscle. In conclusion, our data indicate that: (i) glycogenesis and glycogenolysis can be stimulated concurrently in the skeletal muscle; and (ii) glucose utilization in the skeletal muscle during contraction may be mediated through glycogen turnover.
机译：先前我们已经表明，肾上腺素可以在体内刺激心脏中的糖原合成。我们在这项研究中的目的是确定在增加能量消耗期间骨骼肌糖原合成是否会受到类似影响。电刺激禁食的麻醉大鼠的左坐骨神经，使左后肢肌肉收缩5、10和20分钟。在所有三组大鼠中，电刺激结束时收缩肌中的糖原含量比右后肢的静息肌低约40％。伴随着增强的糖原分解作用，增加了静脉内注入的[3-（3）H]-葡萄糖到糖原中的结合。发现tri气中glyco中糖原的掺入速率是静息肌的34倍。葡萄糖的利用是通过静脉注射骨骼肌中的[14C] -2-脱氧葡萄糖的磷酸化来确定的。发现[14C] -2-脱氧葡萄糖-6-磷酸在收缩肌肉中的蓄积速率是静息肌肉的28倍。在收缩肌肉中未发现糖原合成和葡萄糖摄取指数通过将[3H]-糖原和[14C] -2-DGP中的放射性除以它们各自前体的平均比活而计算得出。 。总之，我们的数据表明：（i）可以同时刺激骨骼肌的糖原生成和糖原分解; （ii）收缩过程中骨骼肌中的葡萄糖利用可能是通过糖原转换来介导的。

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