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首页> 外文期刊>Life sciences >Motilin in human milk: identification and stability during digestion.
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Motilin in human milk: identification and stability during digestion.

机译：母乳中的胃动素：在消化过程中的鉴定和稳定性。

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The presence of motilin in human milk and the influence of human milk on the degradation of [125I][Nle13] porcine motilin by gastric and duodenal fluids were investigated. Milk and plasma samples were collected from 14 mothers, and motilin was measured by radioimmunoassay. Plasma levels were 416 +/- 37 pg/mL. In 8 defatted samples the motilin level was 105 +/- 14 pg/mL, in the six others levels were above 1000 pg/mL but dilution curves were non-linear. After solid-phase extraction milk levels were 108 +/- 21 pg/mL in 13 samples, in 1 sample the dilution curve was still non-linear. The stability of motilin after ingestion was studied in vitro by incubating [121I][Nle13] porcine motilin with gastric and intestinal juices obtained from newborns (10 times diluted). Incubations were performed at 37 degrees C at pH 1.8, 3.2 and 5.8 for the gastric fluid and at pH 7.4 for the duodenal fluid. After different times of intervals (5, 10, 20 and 30 minutes) intact motilin was precipitated with trichloroacetic acid and the radioactivity of the supernatant was determined. Motilin was rapidly degraded by gastric juice. The breakdown was greatest at pH 3.2 (74% after 30 minutes) and lowest at pH 5.8 (29%), the pH after milk feeding in neonates. Degradation by intestinal juice at pH 7.4 was also very rapid (77% after 30 minutes). Human milk and BSA inhibited partially the gastric digestion at pH 3.2 (17 and 29%, respectively). Digestion by intestinal juice was not affected by human milk and BSA. These results suggest that digestion of motilin in the stomach may be sufficiently retarded by human milk in the newborn to exert a biological role.
机译：研究了人乳中胃动素的存在以及人乳对胃液和十二指肠液降解[125I] [Nle13]猪胃动素的影响。从14名母亲那里采集牛奶和血浆样品，并通过放射免疫测定法测定胃动素。血浆水平为416 +/- 37pg / mL。在8个脱脂样品中，胃动蛋白水平为105 +/- 14 pg / mL，在其他六个样品中，胃动蛋白水平为1000 pg / mL以上，但稀释曲线是非线性的。固相萃取后，在13个样品中的牛奶含量为108 +/- 21 pg / mL，在1个样品中，稀释曲线仍然是非线性的。通过将[121I] [Nle13]猪胃动蛋白与新生儿获得的胃液和肠液（稀释10倍）孵育，体外研究了胃动素的摄取稳定性。对于胃液，在37℃，pH 1.8、3.2和5.8下进行孵育;对于十二指肠液，在pH 7.4上进行孵育。在不同时间间隔（5、10、20和30分钟）后，完整的胃动素用三氯乙酸沉淀，并测定上清液的放射性。胃液使胃动素迅速降解。在新生儿喂奶后的pH为3.2时（30分钟后为74％）最大，而pH为5.8（29％）时则最小。 pH 7.4的肠液降解也非常迅速（30分钟后77％）。人乳和BSA在pH 3.2时部分抑制了胃的消化（分别为17％和29％）。肠液消化不受人乳和牛血清白蛋白的影响。这些结果表明，新生儿中的人乳可以充分抑制胃中胃动素的消化，从而发挥生物学作用。

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