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首页> 外文期刊>Life sciences >Anticlastogenic effect of Spirulina maxima extract on the micronuclei induced by maleic hydrazide in Tradescantia.
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Anticlastogenic effect of Spirulina maxima extract on the micronuclei induced by maleic hydrazide in Tradescantia.

机译：极大螺旋藻提取物对Trade虫中马来酰肼诱导的微核的抗成胶作用。

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The aim of this investigation was to determine if extracts of Spirulina maxima reduce the genotoxic damage induced by maleic hydrazide (MH) using the Tradescantia biosssay. Two types of extracts from the alga were prepared: an aqueous extract with two different concentrations, 100 and 500 mg/ml, and a second one, the extract of a 1% solution of dimethyl sulfoxide (DMSO) which corresponded to 100 mg/ml of the alga. The capacity of MH to induce micronuclei (MN) was initially established by administering 0.005, 0.01, and 0.015 mg/ml of the chemical to the Tradescantia inflorescences, and observing its effect after 24 h.The results of this experiment showed a significant MN increase with the two high concentrations tested, although no dose-response effect was observed. For the anticlastogenic assay, the extracts of Spirulina were applied to the inflorescences alone or immediately before the application of MH (0.01 mg/ml) and the induced MN were observed 24 h later. We found that none of the extracts increased the MN level with respect to the untreated plants; also, that MH more or less doubled the basal micronuclei frequency, and finally, that all tested extracts reduced the genotoxic damage caused by MH. The inhibitory indices obtained for the aqueous extracts (100 and 500 mg/ml) and for the DMSO extract were respectively 59, 85, and 56.3%. These data indicate that Spirulina is an anticlastogenic agent and suggest that it is advisable to extend studies on this matter using other biological models.
机译：这项研究的目的是使用Tradescantia生物测定法确定最大螺旋藻提取物是否能降低马来酰肼（MH）引起的遗传毒性损害。制备了两种藻类提取物：一种具有两种不同浓度的水提取物，分别为100和500 mg / ml，另一种为1％二甲基亚砜（DMSO）溶液的提取物，其浓度为100 mg / ml藻类。 MH诱导微核（MN）的能力最初是通过向红scan花中施用0.005、0.01和0.015 mg / ml的化学物质并观察其在24 h后的效果而建立的。本实验的结果表明MN显着增加尽管未观察到剂量反应作用，但在两种高浓度试验中均未发现。为了进行抗成弹性测定，将螺旋藻的提取物单独或在施用MH（0.01 mg / ml）之前施用于花序，并在24 h后观察诱导的MN。我们发现，相对于未处理的植物，没有一种提取物能增加MN含量。同样，MH或多或少使基础微核频率增加了一倍，最后，所有测试提取物均减少了由MH引起的遗传毒性损害。水提取物（100和500 mg / ml）和DMSO提取物的抑制指数分别为59％，85％和56.3％。这些数据表明螺旋藻是一种抗肿瘤药，并建议使用其他生物学模型对该物质进行研究。

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