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首页> 外文期刊>Life sciences >A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury.
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A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury.

机译：从冬虫夏草（一种传统中药）中分离出的多糖可保护PC12细胞免受过氧化氢诱导的伤害。

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摘要

Cordyceps sinensis, a well-known traditional Chinese medicine, possesses activities in anti-tumour, anti-oxidation and stimulating the immune system; however, the identity of active component(s) is not determined. By using anti-oxidation activity-guided fractionation, a polysaccharide of molecular weight approximately 210 kDa was isolated from cultured Cordyceps mycelia by ion-exchange and sizing chromatography. The isolated polysaccharide, having strong anti-oxidation activity, contains glucose, mannose and galactose in a ratio of 1 : 0.6 : 0.75. The pre-treatment of isolated polysaccharide on the cultured rat pheochromocytoma PC12 cells shows strong protective effect against hydrogen peroxide (H(2)O(2))-induced insult. Treatment of the cells with the isolated polysaccharide at 100 microg/ml prior to H(2)O(2) exposure significantly elevated the survival of PC12 cells in culture by over 60%. In parallel, the H(2)O(2)-induced production of malondialdehyde in cultured cells was markedly reduced by the polysaccharide treatment. Moreover, the pre-treatment of the isolated polysaccharide significantly attenuated the changes of glutathione peroxidase and superoxide dismutase activities in H(2)O(2)-treated cells in a dose-dependent manner. This is the first report in identifying a polysaccharide from Cordyceps, which protects against the free radical-induced neuronal cell toxicity.
机译：冬虫夏草是一种著名的中药，具有抗肿瘤，抗氧化和刺激免疫系统的作用。但是，未确定活动组件的身份。通过使用抗氧化活性指导的分馏，通过离子交换和施胶色谱法从培养的虫草菌丝体中分离出分子量约为210 kDa的多糖。具有强的抗氧化活性的分离的多糖含有比例为1∶0.6∶0.75的葡萄糖，甘露糖和半乳糖。培养的大鼠嗜铬细胞瘤PC12细胞上的分离多糖的预处理显示出对过氧化氢（H（2）O（2））诱导的侮辱的强大保护作用。在H（2）O（2）暴露之前，用分离的多糖以100微克/毫升处理细胞，可显着提高培养物中PC12细胞的存活率60％以上。同时，多糖处理显着降低了H（2）O（2）诱导的培养细胞中丙二醛的产生。此外，对分离的多糖的预处理显着减弱了H（2）O（2）处理细胞中谷胱甘肽过氧化物酶和超氧化物歧化酶活性的变化，并呈剂量依赖性。这是从冬虫夏草中鉴定多糖的第一篇报道，该多糖可防止自由基引起的神经元细胞毒性。

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