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首页> 外文期刊>Life sciences >Splenomegaly induced by recombinant human granulocyte-colony stimulating factor in rats.
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Splenomegaly induced by recombinant human granulocyte-colony stimulating factor in rats.

机译：重组人粒细胞集落刺激因子在大鼠中引起脾肿大。

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摘要

Spontaneous ruptures of the spleen have been observed in donors and patients with malignancy during mobilization of peripheral blood stem cells. Thus, we investigated the morphological and histological alteration of the spleen, and mRNA expression levels and activities of the DNA-synthesizing enzymes thymidylate synthase (TS) and thymidine kinase (TK) in the splenic cells, of rats treated with recombinant human granulocyte-colony stimulating factor (rhG-CSF). Daily injections of rhG-CSF for 5 or 7 days slightly enhanced the splenic weight. Single or 3-day treatment with rhG-CSF markedly enhanced the number of granulocytes in peripheral blood. Expression levels of TS and TK mRNA in the splenic cells were significantly increased 6 hours after rhG-CSF treatment. Early expression of TS and TK mRNA in the splenic cells may indicate a reseeding of hematopoietic cells from the bone marrow. Daily injections with rhG-CSF enhanced the TS and TK activities in the splenic cells. As continuous elevations of DNA-synthesizing enzyme activity and spleno-megaly are suggestive of a possible splenic rupture, the monitoring of peripheral granulocytes and splenic size is necessary during the rhG-CSF treatment.
机译：在动员外周血干细胞期间，在供体和恶性肿瘤患者中观察到脾脏自发性破裂。因此，我们研究了用重组人粒细胞集落处理的大鼠脾脏中脾脏的形态学和组织学变化，以及脾脏细胞中DNA合成酶胸苷酸合酶（TS）和胸苷激酶（TK）的mRNA表达水平和活性。刺激因子（rhG-CSF）。每天注射rhG-CSF 5或7天会轻微增加脾脏重量。使用rhG-CSF进行的单日或3天治疗显着增加了外周血中粒细胞的数量。 rhG-CSF处理6小时后，脾细胞中TS和TK mRNA的表达水平显着增加。 TS和TK mRNA在脾细胞中的早期表达可能表明造血细胞从骨髓中重新播种。每天注射rhG-CSF可增强脾细胞的TS和TK活性。由于DNA合成酶活性和脾肿大的持续升高提示可能发生脾破裂，因此在rhG-CSF治疗期间必须监测外周粒细胞和脾大小。

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