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首页> 外文期刊>Life sciences >THE MICROPHOTOHEMOLYTIC RESPONSE OF ERYTHROCYTES IS ALTERED BY STREPTOZOTOCIN-INDUCED DIABETES AND COPPER DEFICIENCY IN RATS
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THE MICROPHOTOHEMOLYTIC RESPONSE OF ERYTHROCYTES IS ALTERED BY STREPTOZOTOCIN-INDUCED DIABETES AND COPPER DEFICIENCY IN RATS

机译：链脲佐菌素诱导的糖尿病和铜缺乏症改变了红细胞的微光溶血反应

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Microphotohemolysis is a new technique that has been used to determine the presence of alterations in the erythrocyte membrane. The method involves light activation through a microscope of a fluorescent dye-erythrocyte-buffer solution in hemocytometer. The interaction of the light and dye result in the generation of toxic oxygen products which attack the membrane allowing water to enter the cell. As hemolysis occurs the optical density of the microscopic field decreases and this is recorded for later quantitation with an image analysis system. Maximal effect, time to half maximal effect and the slope of the hemolysis curve are determined. The goal of this study was to determine if microphotohemolysis could be used to detect differences in erythrocytes from animals with altered physiological states such as hypercholesterolemia, diabetes, and copper deficiency. These are conditions that alter the lipid or protein structure of the erythrocyte membrane and/or the antioxidative capacity of the erythrocyte. There were no effects of hypercholesterolemia on the microphotohemolytic response of the erythrocyte. Streptozotocin-induced diabetes resulted in a decreased maximum effect, a significant shift of the hemolysis curve to the right (increased T 1/2) and a significant decrease in the slope of the hemolysis curve. These results in diabetes and copper deficiency are consistent with an altered protein structure in the erythrocyte membrane that occurs in these conditions. The data demonstrate that this technique may be used to detect differences between normal and altered erythrocytes. As such, it could be useful in monitoring the course of a disease or its treatment. [References: 29]
机译：微光溶血是一种新技术，已用于确定红细胞膜中是否存在改变。该方法包括通过显微镜在血细胞计数器中激活荧光染料-红细胞-缓冲液的光激活。光线和染料的相互作用导致产生有毒的氧气，这些氧气会侵蚀膜，使水进入细胞。随着溶血的发生，显微镜视野的光密度会降低，并记录下来，以供以后使用图像分析系统定量。确定最大作用，达到最大作用一半的时间和溶血曲线的斜率。这项研究的目的是确定微光溶血是否可以用于检测生理状态改变的动物（如高胆固醇血症，糖尿病和铜缺乏症）的红细胞差异。这些是改变红细胞膜的脂质或蛋白质结构和/或红细胞的抗氧化能力的条件。高胆固醇血症对红细胞的微光溶血反应没有影响。链脲佐菌素诱导的糖尿病导致最大效应降低，溶血曲线向右显着偏移（T 1/2升高）和溶血曲线斜率显着下降。这些导致糖尿病和缺铜的结果与在这些情况下发生的红细胞膜蛋白结构改变相一致。数据表明该技术可用于检测正常和改变的红细胞之间的差异。这样，它在监视疾病的进程或其治疗中可能是有用的。 [参考：29]

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