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Sp1 plays an important role in regulating the transcription of ZNF313.

机译:Sp1在调节ZNF313的转录中起重要作用。

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The ZNF313 gene has the highest transcription level in fertile male testes and may be related to human spermatogenesis. The deletion-mutated plasmids of ZNF313 promoter were constructed and transfected into HEK293 cells. The result showed that the fragment from nt -157 to +8 has a basal transcriptional activity. A functional Sp1 binding site was identified by site-directed mutation test and mithramycin A treatment. A 447-bp based at +233 to -213 exhibits a characteristic CpG island, which overlaps with the promoter region. Our work suggests that ZNF313 is controlled at the transcriptional level, and a common mechanism controlling the basal transcription of ZNF313 gene exists.
机译:ZNF313基因在可育雄性睾丸中具有最高的转录水平,可能与人类精子发生有关。构建ZNF313启动子的缺失突变质粒,并将其转染到HEK293细胞中。结果表明,从核苷酸-157到+8的片段具有基础转录活性。通过定向突变试验和光神霉素A处理鉴定了功能性Sp1结合位点。基于+233至-213的447-bp表现出特征性的CpG岛,该岛与启动子区域重叠。我们的工作表明,ZNF313在转录水平受到控制,并且存在控制ZNF313基因基础转录的常见机制。

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