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首页> 外文期刊>Cell biology international. >NADPH oxidase is involved in H 2O 2-induced differentiation of human promyelocytic leukaemia HL-60 cells
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NADPH oxidase is involved in H 2O 2-induced differentiation of human promyelocytic leukaemia HL-60 cells

机译：NADPH氧化酶参与H 2O 2诱导的人早幼粒细胞白血病HL-60细胞分化

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摘要

The expression and activity of NADPH oxidase increase when HL-60 cells are induced into terminally differentiated cells. However, the function of NADPH oxidase in differentiation is not well elucidated. With 150-500 μM H 2O 2 inducing differentiation of HL-60 cells, we measured phagocytosis of latex beads and investigated cell electrophoresis. Two inhibitors of NADPH oxidase, DPI (diphenyleneiodonium) and APO (apocynin), blocked the differentiation potential of cells induced by 200 μM H 2O 2. However, H 2O 2 stimulated the generation of intracellular superoxide (O 2 .-), which decreased in the presence of the two inhibitors. DPI also inhibited H 2O 2-induced ERK (extracellular-signal-regulated kinase) activation, as detected by Western blotting. Furthermore, PD98059, the inhibitor of the ERK pathway, inhibited the differentiation of HL-60 cells induced by H 2O 2. This shows that H 2O 2 can activate NADPH oxidase, leading to O 2 .- production, followed by ERK activation and ultimately resulting in the differentiation of HL-60 cells. The data indicate that NADPH oxidase is an important cell signal regulating cell differentiation.
机译：HL-60细胞被诱导为终末分化细胞后，NADPH氧化酶的表达和活性增加。但是，NADPH氧化酶在分化中的功能尚不清楚。用150-500μMH 2O 2诱导HL-60细胞分化，我们测量了乳胶珠的吞噬作用并研究了细胞电泳。 NADPH氧化酶的两种抑制剂DPI（二苯撑碘鎓）和APO（阿波西宁）阻断了200μMH 2O 2诱导的细胞分化潜能。然而，H 2O 2刺激了细胞内超氧化物（O 2 .-）的生成，并降低了在两种抑制剂的存在下。通过蛋白质印迹检测，DPI还抑制H 2O 2诱导的ERK（细胞外信号调节激酶）活化。此外，ERK通路的抑制剂PD98059抑制了由H 2O 2诱导的HL-60细胞的分化。这表明H 2O 2可以激活NADPH氧化酶，导致O 2 .-生成，然后是ERK激活并最终导致HL-60细胞分化。数据表明NADPH氧化酶是调节细胞分化的重要细胞信号。

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