...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Inhibition of RANKL-dependent cellular fusion in pre-osteoclasts by amiloride and a NHE10-specific monoclonal antibody
【24h】

Inhibition of RANKL-dependent cellular fusion in pre-osteoclasts by amiloride and a NHE10-specific monoclonal antibody

机译：阿米洛利和NHE10特异性单克隆抗体对破骨细胞中RANKL依赖性细胞融合的抑制作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The functions of Na+/H+ exchangers (NHEs) during osteoclastic differentiation were investigated using the NHE inhibitor amiloride and a monoclonal antibody (MAb). Compared with sRANKL-stimulated control cells, amiloride decreased the number of large TRAP-positive osteoclast cells (OCs) with 10 nuclei and increased the number of small TRAP-positive OCs with 10 nuclei during sRANKL-dependent osteoclastic differentiation of RAW264.7 cells. NHE10 mRNA expression and OC differentiation markers were increased by sRANKL stimulation in dose- and time-dependent manners. NHEs 1-9 mRNA expression was not increased by sRANKL stimulation. Similar to amiloride, a rat anti-mouse NHE10 MAb (clone 6B11) decreased the number of large TRAP-positive OCs, but increased the number of small TRAP-positive OCs. These findings suggested that inhibition of NHEs by amiloride or an anti-NHE10 MAb prevented sRANKL-promoted cellular fusion. The anti-NHE10 MAb has the potential for use as an effective inhibitor of bone resorption for targeted bone disease therapy.
机译：使用NHE抑制剂阿米洛利和单克隆抗体（MAb），研究了Na + / H +交换子（NHE）在破骨细胞分化过程中的功能。与sRANKL刺激的对照细胞相比，在RAW264.7细胞的sRANKL依赖性破骨细胞分化过程中，阿米洛利减少了具有10个核的大TRAP阳性破骨细胞（OCs）的数量，并增加了具有10个核的小型TRAP阳性OCs的数量。 sRANKL刺激以剂量和时间依赖性方式增加NHE10 mRNA表达和OC分化标志物。 sRANKL刺激不会增加NHEs 1-9 mRNA的表达。与阿米洛利相似，大鼠抗小鼠NHE10 MAb（克隆6B11）减少了大TRAP阳性OC的数量，但增加了小TRAP阳性OC的数量。这些发现表明，阿米洛利或抗NHE10 MAb对NHE的抑制作用阻止了sRANKL促进的细胞融合。抗NHE10 MAb有潜力用作靶向骨病治疗的有效骨吸收抑制剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号