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首页> 外文期刊>Cell biology international. >Adipose progenitor cells reside among the mature adipocytes: morphological research using an organotypic culture system
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Adipose progenitor cells reside among the mature adipocytes: morphological research using an organotypic culture system

机译：脂肪祖细胞位于成熟的脂肪细胞之间：使用器官型培养系统进行形态学研究

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The precise localization and biological characteristics of the adipose progenitor cells are still a focus of debate. In this study, the localization of the adipose progenitor cells was determined using an organotypic culture system of adipose tissue slices. The tissue slices of subcutaneous white adipose tissue from rats were placed on a porous membrane and cultured at the interface between air and the culture medium for up to 5 days with or without adipogenic stimulation. The structure of adipose tissue components was sufficiently preserved during the culture and, following adipogenic stimulation with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine, numerous multilocular adipocytes appeared in the interstitium among the mature adipocytes. Histomorphological 3-D observation using confocal laser microscopy revealed the presence of small mesenchymal cells containing little or no fat residing in the perivascular region and on the mature adipocytes and differentiation from the pre-existing mesenchymal cells to multilocular adipocytes. Immunohistochemistry demonstrated that these cells were initially present within the fibronectin-positive extracellular matrix (ECM). The adipose differentiation of the mesenchymal cells was confirmed by the enhanced expression of C/EBP- suggesting adipose differentiation and the concurrent advent of CD105-expressing mesenchymal cells within the interstitium of the mature adipocytes. Based on the above, the mesenchymal cells embedded in the ECM around the mature adipocytes were confirmed to be responsible for adipogenesis because the transition of the mesenchymal cells to the stem state contributed to the increase in the number of adipocytes in rat adipose tissue.
机译：脂肪祖细胞的精确定位和生物学特性仍然是争论的焦点。在这项研究中，使用脂肪组织切片的器官型培养系统确定了脂肪祖细胞的定位。将来自大鼠的皮下白色脂肪组织的组织切片放在多孔膜上，在有或没有脂肪形成刺激的情况下，在空气和培养基之间的界面上培养最多5天。在培养过程中，脂肪组织成分的结构得到了充分的保护，在胰岛素，地塞米松和3-异丁基-1-甲基黄嘌呤刺激成脂后，成熟脂肪细胞间质中出现了许多多眼脂肪细胞。使用共聚焦激光显微镜进行的组织形态学3D观察显示，在血管周区域和成熟脂肪细胞上存在少量或不含脂肪的小间充质细胞，并且从先前存在的间充质细胞分化为多房型脂肪细胞。免疫组织化学表明，这些细胞最初存在于纤连蛋白阳性细胞外基质（ECM）中。通过增强C / EBP的表达来证实间质细胞的脂肪分化，这表明脂肪分化和成熟脂肪细胞间质内同时表达CD105的间质细胞的出现。基于上述，证实了在成熟脂肪细胞周围嵌入ECM的间充质细胞是引起脂肪形成的原因，因为间充质细胞向干态的转变有助于大鼠脂肪组织中脂肪细胞数量的增加。

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