...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >In vitro long-term development of cultured inner ear stem cells of newborn rat.
【24h】

In vitro long-term development of cultured inner ear stem cells of newborn rat.

机译：新生大鼠内耳干细胞的体外长期发育。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The adult mammalian auditory receptor lacks any ability to repair and/or regenerate after injury. However, the late developing cochlea still contains some stem-cell-like elements that might be used to regenerate damaged neurons and/or cells of the organ of Corti. Before their use in any application, stem cell numbers need to be amplified because they are usually rare in late developing and adult tissues. The numerous re-explant cultures required for the progressive amplification process can result in a spontaneous differentiation process. This aspect has been implicated in the tumorigenicity of stem cells when transplanted into a tissue. The aim of this study has been to determine whether cochlear stem cells can proliferate and differentiate spontaneously in long-term cultures without the addition of any factor that might influence these processes. Cochlear stem cells, which express nestin protein, were cultured in monolayers and fed with DMEM containing 5% FBS. They quickly organized themselves into typical spheres exhibiting a high proliferation rate, self-renewal property, and differentiation ability. Secondary cultures of these stem cell spheres spontaneously differentiated into neuroectodermal-like cells. The expression of nestin, glial-fibrillary-acidic protein, vimentin, and neurofilaments was evaluated to identify early differentiation. Nestin expression appeared in primary and secondary cultures. Other markers were also identified in differentiating cells. Further research might demonstrate the spontaneous differentiation of cochlear stem cells and their teratogenic probability when they are used for transplantation.
机译：成年哺乳动物的听觉受体在受伤后缺乏任何修复和/或再生的能力。然而，晚期的耳蜗仍包含一些干细胞样成分，可用于再生受损的神经元和/或Corti器官的细胞。在将干细胞用于任何用途之前，必须对其进行扩增，因为干细胞数量通常在晚期发育和成年组织中很少见。渐进扩增过程所需的大量再植培养物可导致自发分化过程。当移植到组织中时，这方面涉及干细胞的致瘤性。这项研究的目的是确定在不添加任何可能影响这些过程的因素的情况下，长期培养的人工耳蜗干细胞是否能自发增殖和分化。将表达巢蛋白的耳蜗干细胞单层培养，并喂以含有5％FBS的DMEM。他们迅速地将自己组织成具有高增殖率，自我更新性和分化能力的典型领域。这些干细胞球的继代培养物自发分化为神经外胚层样细胞。巢蛋白，胶质纤维酸性蛋白，波形蛋白和神经丝的表达进行了评估，以确定早期分化。巢蛋白表达出现在原代和继代培养中。在分化细胞中还鉴定出其他标记。进一步的研究可能表明，当它们用于移植时，耳蜗干细胞会自发分化，并可能致畸。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号