...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >In vitro differentiation of adipose-tissue-derived mesenchymal stem cells into neural retinal cells through expression of human PAX6 (5a) gene
【24h】

In vitro differentiation of adipose-tissue-derived mesenchymal stem cells into neural retinal cells through expression of human PAX6 (5a) gene

机译：通过表达人PAX6（5a）基因，将脂肪组织来源的间充质干细胞体外分化为神经视网膜细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The neural retina is subjected to various degenerative conditions. Regenerative stem-cell-based therapy holds great promise for treating severe retinal degeneration diseases, although many drawbacks remain to be overcome. One important problem is to gain authentically differentiated cells for replacement. Paired box 6 protein (5a) (PAX6 (5a)) is a highly conserved master control gene that has an essential role in the development of the vertebrate visual system. Human adipose-tissue-derived stem cell (hADSC) isolation was performed by using fat tissues and was confirmed by the differentiation potential of the cells into adipocytes and osteocytes and by their surface marker profile. The coding region of the human PAX6 (5a) gene isoform was cloned and lentiviral particles were propagated in HEK293T. The differentiation of hADSCs into retinal cells was characterized by morphological characteristics, quantitative real-time reverse transcription plus the polymerase chain reaction (qPCR) and immunocytochemistry (ICC) for some retinal cell-specific and retinal pigmented epithelial (RPE) cell-specific markers. hADSCs were successfully isolated. Flow cytometric analysis of surface markers indicated the high purity (97 %) of isolated hADSCs. After 30 h of post-transduction, cells gradually showed the characteristic morphology of neuronal cells and small axon-like processes emerged. qPCR and ICC confirmed the differentiation of some neural retinal cells and RPE cells. Thus, PAX6 (5a) transcription factor expression, together with medium supplemented with fibronectin, is able to induce the differentiation of hADSCs into retinal progenitors, RPE cells and photoreceptors.
机译：神经视网膜经受各种退化条件。尽管许多缺点仍有待克服，但基于干细胞的再生疗法有望治疗严重的视网膜变性疾病。一个重要的问题是获得真正分化的细胞进行替换。配对框6蛋白（5a）（PAX6（5a））是高度保守的主控制基因，在脊椎动物视觉系统的发育中具有重要作用。人类脂肪组织衍生的干细胞（hADSC）通过使用脂肪组织进行分离，并通过细胞分化为脂肪细胞和骨细胞的潜力以及它们的表面标志物特征得到证实。克隆人PAX6（5a）基因同工型的编码区，并将慢病毒颗粒在HEK293T中繁殖。 hADSCs分化为视网膜细胞的特征是形态特征，定量实时逆转录结合聚合酶链反应（qPCR）和免疫细胞化学（ICC），用于某些视网膜细胞特异性和视网膜色素上皮细胞（RPE）细胞特异性标记。 hADSC已成功分离。表面标记的流式细胞仪分析表明分离出的hADSCs纯度高（97％）。转导后30 h，细胞逐渐显示神经元细胞的特征形态，并出现小的轴突样过程。 qPCR和ICC证实了某些神经视网膜细胞和RPE细胞的分化。因此，PAX6（5a）转录因子表达以及补充纤连蛋白的培养基能够诱导hADSCs分化为视网膜祖细胞，RPE细胞和感光细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号