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首页> 外文期刊>Limnology >Distribution of dissolved organic carbon and dissolved fulvic acid in mesotrophic Lake Biwa, Japan
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Distribution of dissolved organic carbon and dissolved fulvic acid in mesotrophic Lake Biwa, Japan

机译：日本中营养型琵琶湖中溶解性有机碳和富富酸的分布

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The dissolved organic carbon (DOC) concentrations in mesotrophic Lake Biwa were determined by a total organic carbon (TOC) analyzer, and DOC molecular size distributions were determined by size exclusion chromatography (SEC) using a fluorescence detector at excitation/emission (Ex/Em) levels of 300/425nm with the eluent at pH 9.7. The fluorescence wavelengths for detection were chosen from the result of excitation-emission matrix spectrometry (EEM) analysis for dissolved fulvic acid (DFA) extracted from Ado River (peak A, Ex/Em = 260-270/430-440 nm; peak B, Ex/Em = 300-310/420-430nm). Ado River DFA was eluted with a retention time (RT) of 7.4-8.9 min and the apparent molecular weight was estimated at 22-87 kDa based on the elution curve for the spherical protein molecular weight standard. A DFA peak eluted at the same retention time as Ado River DFA also appeared in all the samples of Lake Biwa water. From the linear relationship between the peak areas with an RT of 7.4-8.9 min by SEC analysis and DOC values of DFA by TOC analysis of a series of DFA samples (r(2) = 0.9995), the concentrations of DFA in the lake water were roughly calculated. DFA was distributed within the range 0.25-0.43 mg Cl-1 and accounted for 15%-41% of DOC, with the highest ratios observed at a depth of 70 m in August and the lowest at 2.5 m in May.
机译：用总有机碳（TOC）分析仪确定了中营养型琵琶湖中溶解的有机碳（DOC）浓度，并使用荧光检测器在激发/发射（Ex / Em）下通过尺寸排阻色谱（SEC）确定了DOC分子大小分布pH 9.7时洗脱液浓度为300 / 425nm）。从Ado River提取的溶解黄腐酸（DFA）的激发发射矩阵光谱（EEM）分析结果中选择用于检测的荧光波长（峰A，Ex / Em = 260-270 / 430-440 nm;峰B ，Ex / Em ＝ 300-310 / 420-430nm）。以7.4-8.9分钟的保留时间（RT）洗脱Ado River DFA，并根据球形蛋白分子量标准品的洗脱曲线估算表观分子量为22-87 kDa。在琵琶湖水的所有样品中都出现了与阿多河DFA保留时间相同的洗脱出的DFA峰。根据一系列DFA样品（r（2）= 0.9995）的SEC分析得出的RT值为7.4-8.9 min的峰面积与TOC分析的DFA DOC值之间的线性关系，湖水中的DFA浓度被粗略计算。 DFA分布在0.25-0.43 mg Cl-1的范围内，占DOC的15％-41％，在8月深度70 m处观察到的比率最高，而在5月2.5m处观察到的比率最低。

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