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Mixture of fibroblast, epithelial and endothelial cells conditioned media induce monocyte-derived dendritic cell maturation.

机译:成纤维细胞,上皮和内皮细胞条件培养基的混合物诱导单核细胞衍生的树突状细胞成熟。

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Fully matured DCs with large amount cytoplasm and copious dendritic projections were visible at the end of culturing period in the presence of MCM, TNF-alpha and poly (I:C), with or without FEECM. Thus, DCs generated with these maturation factors are nonadherent and have typical satellite morphology. Flow cytometric analysis using anti-CD14, -CD80, -CD86, -HLA-DR and -CD83 revealed that expression of CD14 is decreased in particular in FEECM treated DCs, on day 5 and expression of CD80, CD86 and HLA-DR was the higher when FEECM are added to maturation factor. Functionally, when DCs matured in the presence of FEECM elicited stronger MLR, reduced phagocytic activity. These results support the use of the FEECM with MCM, TNF-alpha and poly (I-C) as maturation factor in DC generation that could result in functionally mature monocyte-derived DCs in comparison to either alone.
机译:在存在或不存在FEECM的MCM,TNF-α和poly(I:C)的情况下,在培养期结束时,可以看到具有大量细胞质和大量树突状突起的完全成熟的DC。因此,使用这些成熟因子生成的DC是非粘附的,并且具有典型的卫星形态。使用抗CD14,-CD80,-CD86,-HLA-DR和-CD83的流式细胞仪分析显示,CD14的表达下降,特别是在FEECM处理的DC中,在第5天下降,而CD80,CD86和HLA-DR的表达下降。将FEECM添加到成熟因子时会更高。在功能上,当DC在FEECM存在下成熟时,会引发更强的MLR,从而降低吞噬活性。这些结果支持与MCM,TNF-α和聚(I-C)结合使用的FEECM作为DC生成中的成熟因子,与单独使用它们相比,可能导致功能成熟的单核细胞衍生DC。

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