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首页> 外文期刊>Cellular immunology >Complexin II regulates degranulation in RBL-2H3 cells by interacting with SNARE complex containing syntaxin-3.
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Complexin II regulates degranulation in RBL-2H3 cells by interacting with SNARE complex containing syntaxin-3.

机译：Complexin II通过与含有Syntaxin-3的SNARE复合物相互作用来调节RBL-2H3细胞中的脱颗粒。

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摘要

Recent studies have revealed that SNARE proteins are involved in the exocytotic release (degranulation) in mast cells. However, the roles of SNARE regulatory proteins are poorly understood. Complexin is one such regulatory protein and it plays a crucial role in exocytotic release. In this study, we characterized the interaction between SNARE complex and complexin II in mast cells by GST pull-down assay and in vitro binding assay. We found that the SNARE complex that interacted with complexin II consisted of syntaxin-3, SNAP-23, and VAMP-2 or -8, whereas syntaxin-4 was not detected. Recombinant syntaxin-3 binds to complexin II by itself, but its affinity to complexin II was enhanced upon addition of VAMP-8 and SNAP-23. Furthermore, the region of complexin II responsible for binding to the SNARE complex, was near the central alpha-helix region. These results suggest that complexin II regulates degranulation by interacting with the SNARE complex containing syntaxin-3.
机译：最近的研究表明，SNARE蛋白参与肥大细胞的胞吐释放（脱粒）。但是，对SNARE调节蛋白的作用了解甚少。复杂蛋白是一种这样的调节蛋白，它在胞吐释放中起关键作用。在这项研究中，我们通过GST下拉测定法和体外结合测定法表征了SNARE复合物和肥大细胞中复合物II之间的相互作用。我们发现与complexin II交互作用的SNARE复合体由语法in-3，SNAP-23和VAMP-2或-8组成，而未检测到语法in-4。重组syntaxin-3自身与complexin II结合，但是通过添加VAMP-8和SNAP-23可以增强其对complexin II的亲和力。此外，负责与SNARE复合物结合的复合物II区域靠近中央α-螺旋区域。这些结果表明，复合物II通过与包含syntaxin-3的SNARE复合物相互作用来调节脱粒。

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