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首页> 外文期刊>Cellular immunology >Effect of double-stranded DNA on maturation of dendritic cells in vitro.
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Effect of double-stranded DNA on maturation of dendritic cells in vitro.

机译：双链DNA体外对树突状细胞成熟的影响。

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摘要

A preparation of human genomic fragmented double-stranded DNA (dsDNA) was used as maturation stimulus in cultures of human dendritic cells (DCs) generated in compliance with the interferon protocol. Culturing of the DCs in medium with 5mug/ml of the DNA preparation was associated with a decrease in the relative proportion of CD14 + cells and an increase in that of CD83 + cells. These changes are markers of DC maturation. The efficiency with which the DNA preparation was able to elicit DC maturation was commensurate with that of lypopolysaccharide from bacterial cell, the standard inducer of DC maturation. Generated ex vivo, matured in the presence of the human DNA preparation, pulsed with tumor antigens mouse DCs were used as a vaccine in biological tests for its antitumor activity. The experimental results demonstrate that reinfusion of mature pulsed with tumor antigens DCs cause a statistically significant suppression of tumor graft growth.
机译：人类基因组片段化双链DNA（dsDNA）的制备物被用作在按照干扰素方案产生的人树​​突状细胞（DC）培养物中的成熟刺激物。用5mug / ml DNA制剂在培养基中培养DC与CD14 +细胞相对比例的减少和CD83 +细胞相对比例的增加有关。这些变化是DC成熟的标志。 DNA制备物能够引发DC成熟的效率与细菌细胞（DC成熟的标准诱导剂）中的脂多糖的效率相当。离体产生的，在人DNA制备物存在下成熟，用肿瘤抗原脉冲的小鼠DCs用作抗肿瘤活性的生物学测试疫苗。实验结果表明，用肿瘤抗原DC重新注入成熟的脉冲后，在统计学上显着抑制了肿瘤移植物的生长。

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