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首页> 外文期刊>Cell and Tissue Research >Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2
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Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2

机译：Tob1在发育中的性腺和成年性腺中表达，并与P体标记Dcp2相关

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Tob1 is a member of the BTG/TOB family of proteins with established antiproliferative function. In Danio rerio and Xenopus laevis, the Tob1 gene is expressed from the one-cell stage through to early gastrula stages, followed in later development by discrete expression in many tissues including the notochord and somites. In both mouse and human, Tob1 is expressed in many adult tissues including the testis and ovary; however, the specific cell types are unknown. We examine Tob1 gene expression in mouse in developing germ cells and in sorted male germ cells (gonocytes, spermatogonia, pachytene spermatocytes and round spermatids) by reverse transcription and droplet digital polymerase chain reaction (RT-ddPCR) and in adult ovary and testis by immunofluorescence with anti-Tob1 protein staining. By RT-ddPCR, Tob1 expression was low in developing male germ cells but was highly expressed in round spermatids. In developing female germ cells undergoing entry into meiosis, it increased 10-fold. Tob1 was also highly expressed in round spermatids and in oocytes in all stages of folliculogenesis. Notably, a marker for P-bodies, Dcp-2, was also highly expressed in round spermatids and all oocyte stages examined. The cytoplasmic presence of Tob1 protein in round spermatids and oocytes and the association of Tob1 protein with Dcp2 in both cell types suggest that Tob1 protein plays a role in post-transcriptional mechanisms.
机译：Tob1是BTG / TOB蛋白质家族的成员，具有确定的抗增殖功能。在Danio rerio和Xenopus laevis中，Tob1基因从单细胞阶段到早期胃胚期表达，随后在后来的发展中在包括脊索和体节在内的许多组织中离散表达。在小鼠和人类中，Tob1在许多成年组织中表达，包括睾丸和卵巢。但是，具体的细胞类型未知。我们通过逆转录和液滴数字聚合酶链反应（RT-ddPCR）以及通过免疫荧光法检测发育中的生殖细胞和分类的雄性生殖细胞（生殖细胞，精原细胞，粗线精子细胞和圆形精子细胞）中Tob1基因的表达与抗Tob1蛋白染色。通过RT-ddPCR，Tob1表达在发育中的雄性生殖细胞中低，但在圆形精子细胞中高表达。在发育成减数分裂的雌性生殖细胞中，它增加了10倍。在卵泡形成的所有阶段，Tob1在圆形精子和卵母细胞中也高度表达。值得注意的是，P抗体的标记物Dcp-2在圆形精子细胞和所有卵母细胞阶段也都高度表达。圆形精子和卵母细胞中Tob1蛋白在细胞质中的存在以及两种细胞类型中Tob1蛋白与Dcp2的关联都表明Tob1蛋白在转录后机制中起作用。

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