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Molecular characterization of two cultivars of Egyptian clover (Trifolium alexandrinum L.).

机译:埃及三叶草的两个品种的分子特征( Trifolium alexandrinum L.)。

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Two Egyptian clover cultivars namely 'Fahl' and 'Helaly' were analyzed based on seed soluble protein pattern as well as RAPD, ISSR and AFLP generated DNA profiles. For AFLP analysis, DNA of Egyptian clover cultivars were digested with EcoRI+MseI restriction enzymes. Among the molecular markers used ISSR showed highest level of molecular variance (24.5%). In terms of allele frequency (p), level of difference between two cultivars was variable. Results also indicated that relationship study between two cultivars can be better performed using Cubic, Quadratic model.
机译:根据种子可溶性蛋白模式以及RAPD,ISSR和AFLP生成的DNA谱,分析了两个埃及三叶草品种'Fahl'和'Helaly'。为了进行AFLP分析,将埃及三叶草品种的DNA用EcoRI + MseI限制性酶消化。在使用的分子标记中,ISSR显示出最高的分子变异水平(24.5%)。就等位基因频率(p)而言,两个品种之间的差异水平是可变的。结果还表明,使用三次方模型可以更好地进行两个品种之间的关系研究。

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