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首页> 外文期刊>Cell cycle >Distinct dynamics of Aurora B and Survivin during mitosis.
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Distinct dynamics of Aurora B and Survivin during mitosis.

机译：在有丝分裂期间Aurora B和Survivin的不同动态。

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摘要

We have studied the dynamics of Aurora B and Survivin during mitosis in living cells, using C-terminal GFP chimeras of the two proteins. These chimeras showed identical localization and behave as bona fide wild type proteins. The mobility of Aurora B-GFP and Survivin-GFP was analyzed by FRAP. The data show that Survivin-GFP, in contrast to Aurora B-GFP, is highly mobile at prometaphase and metaphase. At telophase and cell cleavage, both chimeras are found to be fully immobile. The ablation of Aurora B by siRNA results in a dramatic decrease of the Survivin-GFP mobility. These results demonstrate that Survivin, but not Aurora B, is weakly associated with the centromeric chromatin at prometaphase and metaphase. The weak association of Survivin with centromeric chromatin is dependent on the presence of Aurora B and is not affected by treatment with either nocodazole or taxol. The rapid and conditional interchange between passenger proteins that we show by live imaging indicates that the high affinity interactions demonstrated with in vitro analysis of passenger protein binding are, in fact, static "snapshots" of highly dynamic and regulated in vivo interactions in mitotic cells.
机译：我们已经使用两种蛋白质的C端GFP嵌合体研究了活细胞有丝分裂期间Aurora B和Survivin的动力学。这些嵌合体显示相同的定位，并表现为真正的野生型蛋白。 FRAP分析了Aurora B-GFP和Survivin-GFP的迁移率。数据显示，与Aurora B-GFP相比，Survivin-GFP在前中期和中期具有很高的移动性。在末期和细胞分裂时，发现两个嵌合体是完全不动的。 siRNA切除Aurora B会导致Survivin-GFP迁移率急剧下降。这些结果表明，Survivin，而不是Aurora B，在前中期和中期与着丝粒染色质弱相关。 Survivin与着丝粒染色质的弱关联取决于Aurora B的存在，不受诺考达唑或紫杉醇治疗的影响。我们通过实时成像显示的客运蛋白质之间的快速和有条件的交换表明，客运蛋白质结合的体外分析表明，高亲和力相互作用实际上是有丝分裂细胞中高动态且受调节的体内相互作用的静态“快照”。

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