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首页> 外文期刊>Cell cycle >Cyclin mRNA stability does not vary during the cell cycle.
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Cyclin mRNA stability does not vary during the cell cycle.

机译：细胞周期蛋白的细胞周期蛋白mRNA稳定性不变。

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摘要

The cyclins are tightly regulated elements governing eukaryotic cell cycle progression by means of sequential activation-inactivation of cyclin-dependent kinases. In one manifestation of this regulation, the mRNA levels of several cyclin genes oscillate during the cycle in mammalian cells. Such cycle-dependent fluctuations in transcript levels could result from changes not only in rates of transcription, but also in mRNA stability. Here we used a new, minimally-disturbing method for producing multi-cycle synchronous growth of human MOLT-4 cells, in combination with quantitative real-time RT-PCR, to compare cell cycle-dependent transcript levels and half-lives of cyclin A2, B1, D3, E and PCNA mRNAs. While all mRNA levels except cyclin D3 varied in the cycle, there were no apparent variations in message half-lives. This differs from several previous reports of dramatic fluctuations in the stabilities of cyclin mRNAs, and infers that fluctuations in cyclin mRNA transcript levels during the MOLT-4 cell cycle are not due to variations in half-lives. The discrepancy in mRNA stability determinations could be due to differences in cell types or synchronization methods, but our findings may be representative of mRNA processing in the cycle of cells in unstressed steady-state growth.
机译：细胞周期蛋白是通过细胞周期蛋白依赖性激酶的顺序激活-失活来调控真核细胞周期进程的严格调控的元件。这种调节的一种表现是，在哺乳动物细胞的周期中，几种细胞周期蛋白基因的mRNA水平振荡。转录水平的这种周期依赖性波动不仅可以由转录速率的变化引起，而且可以由mRNA稳定性的变化引起。在这里，我们使用一种新的，干扰最小的方法来产生人类MOLT-4细胞的多周期同步生长，并与定量实时RT-PCR相结合，比较了细胞周期依赖性转录水平和细胞周期蛋白A2的半衰期，B1，D3，E和PCNA mRNA。尽管除周期蛋白D3以外的所有mRNA水平都在周期中变化，但消息半衰期没有明显变化。这不同于先前关于细胞周期蛋白mRNA稳定性剧烈波动的几篇报道，并推断出在MOLT-4细胞周期中细胞周期蛋白mRNA转录水平的波动并不是由于半衰期的变化。 mRNA稳定性测定中的差异可能是由于细胞类型或同步方法的差异所致，但我们的发现可能代表无应力稳态生长中细胞周期中mRNA的加工。

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