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首页> 外文期刊>Livestock Science >Effect of testosterone, estrone sulphate and androstenone on 3 beta -hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes.
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Effect of testosterone, estrone sulphate and androstenone on 3 beta -hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes.

机译：睾丸激素，硫酸雌酮和雄烯酮对原代培养肝细胞中3β-羟类固醇脱氢酶蛋白表达的影响。

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摘要

Androstenone (5-androst-16-en-3-one) is a steroid pheromone, excessive accumulation of which in pig adipose tissue contributes to the phenomenon of "boar taint". The incidence of boar taint increases with age and weight of entire male pigs. One of the reasons for the excessive accumulation of androstenone in adipose tissue of some pigs is a low rate of androstenone degradation in pig liver, which is related to defective expression of the androstenone-metabolising enzyme 3 beta -hydroxysteroid dehydrogenase (3 beta -HSD). The mechanism regulating 3 beta -HSD expression in pig liver remains unclear. The present study investigated the effects of the sex steroids testosterone, estrone sulphate and androstenone on the expression of 3 beta -HSD protein using primary cultured hepatocytes as a model system. The study was performed on hepatocytes from pigs of two weight/age groups: "lighter weight" (average carcass weight 70 kg, age 21 weeks) and "heavier weight" (average carcass weight 92 kg, age 26 weeks). Testosterone at the range of concentrations from 5 to 100 nM induced 3 beta -HSD expression in "lighter weight" pigs but not in "heavier weight" animals. In contrast, estrone sulphate (5 to 100 nM) and androstenone (5 to 500 nM) induced 3 beta -HSD expression only in "heavier weight" but not "lighter weight" pigs. Induction of 3 beta -HSD expression by the sex steroids was protein-synthesis-dependent. Overall this study demonstrates for the first time that sex steroids are involved in regulation of 3 beta -HSD protein expression in primary cultured pig hepatocytes and that the effect of sex steroids depends on age and weight of animals. The results of this work might contribute to understanding the mechanism controlling androstenone deposition in pigs.
机译：雄烯酮（5-androst-16-en-3-one）是一种类固醇信息素，其在猪脂肪组织中的过度积累导致了“公猪异味”现象。公猪异味的发生率随整个公猪的年龄和体重而增加。某些猪的脂肪组织中雄烯酮的过量积累的原因之一是猪肝脏中雄烯酮的降解率低，这与雄烯酮代谢酶3β-羟类固醇脱氢酶（3β-HSD）的表达缺陷有关。 。调节猪肝脏中3β-HSD表达的机制尚不清楚。本研究使用原代培养的肝细胞作为模型系统，研究了性类固醇睾丸激素，硫酸雌酮和雄烯酮对3β-HSD蛋白表达的影响。该研究是针对两个体重/年龄组的猪的肝细胞进行的：“体重较轻”（平均cas体体重70公斤，年龄21周）和“体重较重”（平均cas体体重92公斤，年龄26周）。浓度范围为5到100 nM的睾丸激素在“体重较轻”的猪中诱导3β-HSD表达，而在“体重较重”的动物中不诱导。相反，硫酸雌酮（5至100 nM）和雄烯酮（5至500 nM）仅在“体重较重”的猪中诱导3β-HSD表达。性类固醇诱导3β-HSD表达是蛋白质合成依赖性的。总体而言，这项研究首次证明性类固醇参与了原代培养猪肝细胞中3β-HSD蛋白表达的调节，而性类固醇的作用取决于动物的年龄和体重。这项工作的结果可能有助于了解控制猪中雄烯酮的沉积机理。

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