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Effect of forage conservation method on microbial flora and fermentation pattern in forage and in equine colon and faeces

机译:饲草保存方法对饲草以及马结肠和粪便中微生物菌群和发酵方式的影响

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The influence of forage conservation method on microbial and chemical composition in forage and on colon content and faeces in horses fed with the forages was investigated. Hay (815 g DM/kg), haylage (548 g DM/kg) and silage (343 g DM/kg) was produced from the same grass crop and fed to four fistulated horses in a crossover study. Horses were sampled in right ventral colon and of faeces after a feeding period of 21 days (for each forage). Fermentation kinetics of right ventral colon was also studied on two subsequent days in each period, with sampling times immediately before and 2, 4, 8 and 12 h after the morning meal. Forage conservation method influenced microbial and chemical composition of the forages. Silage differed from haylage and hay in more variables than haylage differed from hay. However, the different forage types had similar influence on microbial and chemical composition of right ventral colon content and faeces in horses at Day 21, with the exception of counts of Streptococci which were highest when horses were fed with hay. However, interaction among forage types and sampling locations (right ventral colon and faeces) was also present for Streptococci as well as for pH. In general, pH was lower and counts of lactate utilizing and lactate producing bacteria were higher in faeces compared to right ventral colon. Fermentation kinetics in colon varied little among forage types and sampling times, and no interaction between forage types and sampling times were found, indicating that silage, haylage and hay had very similar fermentation kinetics in right ventral colon of horses. In conclusion, silage, haylage and hay produced from the same grass crop tended to produce similar responses in microbial and chemical composition in right ventral colon and faeces in horses fed with the forages.
机译:研究了饲草保存方法对饲草中微生物和化学成分以及饲草中马的结肠含量和粪便的影响。干草(815 g DM / kg),海杂粮(548 g DM / kg)和青贮饲料(343 g DM / kg)是从同一草料作物生产的,并在交叉研究中喂给四头f马。在21天的饲喂期后(对每种饲料),在右腹结肠和粪便中对马取样。在每个时段的后两天,还研究了右腹结肠的发酵动力学,采样时间分别为早饭前后,饭后2、4、8和12 h。饲草保存方法影响了饲草的微生物和化学组成。青贮饲料与海草饲料和干草的区别在于,青贮饲料与干草和干草的区别更大。但是,在第21天,不同的饲草类型对马的右腹结肠含量和粪便的微生物和化学组成具有相似的影响,但链球菌的计数除外,当饲喂干草时,链球菌的计数最高。但是,链球菌和pH值也存在牧草类型和采样位置(右腹结肠和粪便)之间的相互作用。一般而言,与右腹结肠相比,粪便中的pH值较低,并且利用乳酸和产生乳酸的细菌的数量较高。在饲草类型和取样时间之间,结肠的发酵动力学变化不大,并且在饲草类型和取样时间之间没有相互作用,这表明青贮饲料,青贮饲料和干草在马的右腹结肠中具有非常相似的发酵动力学。总之,从同一草类作物中收获的青贮饲料,干草和干草倾向于在饲喂饲草的马的右腹结肠和粪便中产生相似的微生物和化学成分响应。

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