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Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits

机译：估计成年兔子食in摄入的替代方法

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This study was carried out in order to estimate caecotrophe intake in growing rabbits by three existing procedures: caecotrophes collection after collar fitting, urinary purine derivatives (PD) excretion and microbial p#eN-lysine incorporation. In a first experiment sixteen New Zealand White male rabbits were divided in three groups receiving the same diet, but supplemented with p#eNHCl in the first group (T: 6 rabbits). The second group (T: 6 rabbits) was also fed the labelled diet but only during the last ten days of the fattening period when animals were fitted a neck collar to prevent caecotrophy. The third group (T: 4 animals) received the basal diet and was used as control. In two additional trials the daily contribution to urinary excretion of endogenous purine compounds (469pl50.8 omol/W."e) and creatinine excretion (807pl127.6 omol/W."e) were determined. The highest estimation of microbial nitrogen recycling was obtained by the urinary PD method (0.79pl0.096 g/d), whereas caecotrophes collection and p#eN-lysine incorporation methods showed similar values (0.49pl0.049 and 0.45pl0.015 g/d, respectively). Our results seem to indicate an overestimation of microbial nitrogen recycling in growing rabbits by PD methodology, while neck collar fitting procedure gave similar results, although more variable than microbial p#eN-lysine incorporation.
机译：进行这项研究是为了通过三种现有的方法来估计生长中的家兔的盲肠摄入量：套环安装后的盲肠收集，尿嘌呤衍生物（PD）排泄和微生物p＃eN-赖氨酸的掺入。在第一个实验中，将16只新西兰白雄性兔分为三组，每组接受相同的饮食，但在第一组中补充p＃eNHCl（T：6只兔）。第二组（T：6只兔子）也饲喂了标记的饮食，但仅在育肥期的最后十天中，即为动物安装颈环以防止盲肠。第三组（T：4只动物）接受基础饮食并用作对照。在另外两个试验中，确定了内源性嘌呤化合物（469pl50.8 omol / W。” e）和尿肌酐排泄（807pl127.6 omol / W。” e）对尿排泄的每日贡献。尿PD法（0.79pl0.096 g / d）获得了最高的微生物氮再循环估算，而e骨收集和p＃eN-赖氨酸掺入法显示相似的值（0.49pl0.049和0.45pl0.015 g / d）。 d）。我们的结果似乎表明通过PD方法高估了成长中兔子体内微生物氮的再循环，尽管颈环拟合过程比微生物p＃eN-赖氨酸的掺入更具可变性，但颈项拟合过程却得出了相似的结果。

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