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首页> 外文期刊>Cellular immunology >The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).
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The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).

机译：酶原颗粒蛋白2（GP2）与内皮细胞I（SREC-1）上表达的清道夫受体结合。

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摘要

The pancreatic zymogen granule membrane protein (GP2) is expressed by pancreatic acinar cells and M cells of the ileum. GP2 is the closest related homologue of the urine resident Tamm-Horsfall protein (THP). Recently, it was shown that THP is a ligand of various scavenger receptors (SRs). Therefore, we were interested, if GP2 has similar properties. cDNA of different SRs was stably transfected into a murine thymoma cell line. GP2 was recombinantly expressed, purified and biotinylated. Binding or uptake of GP2 by transfected cells or monocyte-derived dendritic cells (moDCs) was analyzed by flow-cytometry. GP2 is a binding partner of the scavenger receptor expressed on endothelial cells I (SREC-I) but not of SR-AI and SR-BI. The dissociation constant (K(d)) of GP2 binding to SREC-I is 41.3nM. SREC transfected cells are able to internalize GP2. moDCs express SREC-I and also bind and internalize GP2. Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding. Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response. In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.
机译：胰酶原颗粒膜蛋白（GP2）由回肠的胰腺腺泡细胞和M细胞表达。 GP2是尿液驻留Tamm-Horsfall蛋白（THP）的最接近相关同源物。最近，显示THP是各种清除剂受体（SR）的配体。因此，我们对GP2是否具有相似的特性感兴趣。将不同SR的cDNA稳定转染到鼠胸腺瘤细胞系中。 GP2被重组表达，纯化和生物素化。通过流式细胞术分析了转染的细胞或单核细胞衍生的树突状细胞（moDC）对GP2的结合或摄取。 GP2是在内皮细胞I（SREC-1）上表达的清道夫受体的结合伴侣，但不是SR-AI和SR-BI的伴侣。与SREC-1结合的GP2的解离常数（K（d））为41.3nM。 SREC转染的细胞能够内化GP2。 moDC表达SREC-1，并结合并内化GP2。用抗SREC-1抗体抑制moDC上的SREC-1不会导致GP2结合减少。 GP2与SREC-1的相互作用和摄取可能对抗原清除和免疫应答介导有深远影响。除了SREC-1外，DC上GP2的其他目前未知受体也可能参与此过程。

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