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首页> 外文期刊>Livestock Science >GnRH agonist active immunization influences ovarian development and GnRH receptor mRNA expression levels of pituitary in Japanese white rabbits (Oryctolagus cuniculus)
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GnRH agonist active immunization influences ovarian development and GnRH receptor mRNA expression levels of pituitary in Japanese white rabbits (Oryctolagus cuniculus)

机译：GnRH激动剂主动免疫会影响日本白兔（Oryctolagus cuniculus）卵巢发育和垂体GnRH受体mRNA表达水平

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The present study was conducted to assess the effects of active immunization against the gonadotropin-releasing hormone agonist (GnRHa) on ovary development and GnRH receptor mRNA expression levels in pituitary in Japanese white rabbits (Oryctolagus cuniculus). Twenty-four three-month-old rabbits were randomly divided into four groups (n=6). The animals were subcutaneously injected with 100 mu g of GnRHa (alarelin) antigen in experimental group I (EG-I), 100 mu g in experimental group II (EG-II) and 50 mu g in experimental group III (EG-III) respectively. Alarelin antigens were re-injected in EG-II and EG-III with the same dosage on 20 days. CG was blank. The samples of ovaries and uteri were collected aseptically at the end of the experiment (70 days). Tissue slices were observed under light and electron microscopes. Serum anti-GnRH antibody titers and concentrations of FSH and LH were measured with ELISA. The anti-GnRH antibody titers reached the peak levels respectively at 30 days in EG-I (1:800), at 40-50 days in EG-II (1:1600) and at 40-50 days in EG-III (1:800). From 40 to 70 days, anti-GnRH antibody titers in EG-II were higher than that in EG-I and EG-III (p<0.05). The highest FSH levels in EG-II and EG-III were detected on 40 days. FSH level in EG-II was higher than those in EG-I, CG (p<0.01) and EG-III (p<0.05) on 40 days. There was no significant difference in FSH levels between EG-I and CG. Serum LH concentrations in EG-II and EG-III reached the peak levels on 50 days and 40 days, LH level in EG-II exceeded other 3 groups on 50 days (p<0.05). Real-time quantitative PCR analysis indicated that the levels of FSH-beta mRNA and GnRH-R mRNA in pituitary significantly declined, while LH-beta mRNA increased at 70 days following alarelin antigen treatment. The results also showed that alarelin antigen treatment improves the development of ovaries, follicles and uteri in Japanese white rabbits. Alarelin antigen treatment and re-injection with 100 mu g dose had better effects than 50 mu g treatment. This may have significance for the development of novel GnRH-based techniques in regulative functions in rabbits. Crown Copyright
机译：本研究旨在评估针对促性腺激素释放激素激动剂（GnRHa）的主动免疫对日本白兔（Oryctolagus cuniculus）垂体卵巢发育和GnRH受体mRNA表达水平的影响。将24只3个月大的兔子随机分为四组（n = 6）。在实验组I（EG-I）中为动物皮下注射100μg GnRHa（alarelin）抗原，在实验组II（EG-II）中向动物皮下注射100μg，在实验组III（EG-III）中为50μg分别。在20天的相同剂量下，将Alarelin抗原重新注入EG-II和EG-III中。 CG为空白。在实验结束时（70天）以无菌方式收集卵巢和子宫样品。在光学和电子显微镜下观察组织切片。用ELISA测量血清抗GnRH抗体滴度和FSH和LH浓度。抗GnRH抗体效价分别在EG-I（1：800）30天，EG-II（1：1600）40-50天和EG-III 40-50天达到峰值（1 ：800）。从40天到70天，EG-II中的抗GnRH抗体滴度高于EG-I和EG-III（p <0.05）。在40天时，在EG-II和EG-III中检测到最高的FSH水平。 EG-II的FSH水平在40天时高于EG-I，CG（p <0.01）和EG-III（p <0.05）。 EG-1和CG之间的FSH水平无显着差异。 EG-II和EG-III的血清LH浓度在50天和40天达到峰值，而EG-II的LH水平在50天超过其他3组（p <0.05）。实时定量PCR分析表明，在alarelin抗原治疗后70天，垂体中FSH-βmRNA和GnRH-R mRNA的水平显着下降，而LH-βmRNA升高。结果还表明，阿拉瑞林抗原治疗可改善日本白兔卵巢，卵泡和子宫的发育。阿拉瑞林抗原治疗和100μg剂量的再注射比50μg的治疗效果更好。这可能对开发新的基于GnRH的家兔调节功能具有重要意义。皇冠版权

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