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首页> 外文期刊>Cellular immunology >Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages
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Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages

机译：脂多糖诱导人中性粒细胞和单核细胞衍生巨噬细胞中五环素3的表达

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摘要

Pentraxin 3 (PTX3) is a prototype protein of long pentraxin. PTX3 is produced by various cells, such as monocytes/macrophages (M phi s) in response to lipopolysaccharide (LPS) and proinflammatory signals. We performed immunoblotting, immunohistochemical staininig. reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA) of PTX3 in human monocyte-derived M phi s and neutrophils. PTX3 expression was observed in the cytoplasm of both GM-CSF induced monocyte-derived M phi (GM-M phi) and M-CSF induced monocyte-derived M phi (M-M phi). PTX3 level in both M phi s was up-regulated at 24 It after LPS stimulation. Moreover, we confirmed PTX3 expression in freshly isolated neutrophils, and PTX3 level was distinctly up-regulated at 6 and 24 It after LPS stimulation. These findings suggested that PTX3 expression, not only in M phi s, but also in neutrophils, may reflect the role of PTX3 in inflammation. We believe that PTX3 can contribute as a diagnostic tool to evaluate inflammation at peripheral sites. (C) 2007 Elsevier Inc. All rights reserved.
机译：Pentraxin 3（PTX3）是长pentraxin的原型蛋白。 PTX3由各种细胞产生，例如单核细胞/巨噬细胞（M phi s）对脂多糖（LPS）和促炎信号的响应。我们进行了免疫印迹，免疫组织化学染色。人单核细胞衍生的M phis和嗜中性粒细胞中PTX3的逆转录酶-聚合酶链反应（RT-PCR），实时定量PCR和酶联免疫吸附测定（ELISA）。在GM-CSF诱导的单核细胞衍生的M phi（GM-M phi）和M-CSF诱导的单核细胞衍生的M phi（M-M phi）的细胞质中均观察到PTX3表达。 LPS刺激后，两个M phi中的PTX3水平均在24 It上调。此外，我们证实了PTX3在新鲜分离的嗜中性粒细胞中的表达，并且LPS刺激后6和24 It，PTX3水平明显上调。这些发现表明，PTX3表达不仅在M phis中，而且在嗜中性粒细胞中也可能反映了PTX3在炎症中的作用。我们认为PTX3可以作为诊断工具来评估周围部位的炎症。 （C）2007 Elsevier Inc.保留所有权利。

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