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首页> 外文期刊>Cell and Tissue Research >Uptake and transport of foreign particles in Peyer's patches of both distal ileum and jejunum of calves.
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Uptake and transport of foreign particles in Peyer's patches of both distal ileum and jejunum of calves.

机译：回肠远端和小肠空肠的Peyer斑中异物的摄取和运输。

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To investigate the uptake and transport patterns of variously sized particles in Peyer's patches (PPs) of calves, intestinal loops were created in four newborn and two 2-month-old calves, and the loops were inoculated with various particles, including carbon black, fluorescein isothiocyanate (FITC)-labeled latex, FITC-labeled dextran, bovine serum, and recombinant mouse prion protein (rMPrP). The intestinal loops were recovered at 3, 6, 9, and 24 h in newborn calves and at 24 h in 2-month-old calves after inoculation, and the transport of the particles was examined by histological and immunohistochemical means. The uptake of the particles was quantified by estimation of signal intensities. A greater intensity was found in newborn calves compared with the 2-month-old calves. The peak uptake of carbon black, FITC-labeled latex, and rMPrP in the PPs of the distal ileum occurred at 6 h after inoculation in newborn calves and then progressively decreased with time. Uptake was also dependent on the site within the small intestine and the size of the particle studied. The transport of carbon black, FITC-labeled latex, and FITC-labeled dextran occurred via the bloodstream, the mesenteric lymph nodes, and the liver of newborn calves. rMPrP was found primarily in the interfollicular regions of the submucosa of the distal ileum of newborn calves. Thus, distal ileal PPs are probably more effective at particle absorption than the jejunal PPs, and the peak uptake of the PPs within the newborn calf occurs at 6 h after inoculation.
机译：为了研究小牛派伊尔氏淋巴集结（PPs）中各种大小颗粒的吸收和运输方式，在四只新生小牛和两只两个月大的小牛中创建了肠loop，并在这些小in中接种了各种颗粒，包括炭黑，荧光素异硫氰酸酯（FITC）标记的乳胶，FITC标记的葡聚糖，牛血清和重组小鼠病毒蛋白（rMPrP）。接种后，新生小牛在3、6、9和24小时以及2个月大的小牛在24小时时恢复了肠loop，并通过组织学和免疫组织化学方法检查了颗粒的转运。通过估计信号强度来量化颗粒的吸收。与两个月大的犊牛相比，新生犊牛的强度更高。新生小牛接种后6 h，回肠末端PPs中炭黑，FITC标记的乳胶和rMPrP的吸收峰值出现，然后随时间逐渐降低。摄取也取决于小肠内的部位和所研究颗粒的大小。炭黑，FITC标记的乳胶和FITC标记的葡聚糖的运输是通过血液，肠系膜淋巴结和新生小牛的肝脏进行的。 rMPrP主要在新生小牛回肠远端粘膜下的小泡间区域发现。因此，回肠远端PPs可能比空肠PPs更有效地吸收颗粒，并且新生小腿内PPs的吸收高峰发生在接种后6 h。

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