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首页> 外文期刊>Cell and Tissue Research >Smooth-muscle-specific expression of neurotrophin-3 in mouse embryonic and neonatal gastrointestinal tract.
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Smooth-muscle-specific expression of neurotrophin-3 in mouse embryonic and neonatal gastrointestinal tract.

机译：小鼠胚胎和新生儿胃肠道中Neurotrophin-3的平滑肌特异性表达。

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Vagal gastrointestinal (GI) afferents are essential for the regulation of eating, body weight, and digestion. However, their functional organization and the way that this develops are poorly understood. Neurotrophin-3 (NT-3) is crucial for the survival of vagal sensory neurons and is expressed in the developing GI tract, possibly contributing to their survival and to other aspects of vagal afferent development. The identification of the functions of this peripheral NT-3 thus requires a detailed understanding of the localization and timing of its expression in the developing GI tract. We have studied embryos and neonates expressing the lacZ reporter gene from the NT-3 locus and found that NT-3 is expressed predominantly in the smooth muscle of the outer GI wall of the stomach, intestines, and associated blood vessels and in the stomach lamina propria and esophageal epithelium. NT-3 expression has been detected in the mesenchyme of the GI wall by embryonic day 12.5 (E12.5) and becomes restricted to smooth muscle and lamina propria by E15.5, whereas its expression in blood vessels and esophageal epithelium is first observed at E15.5. Expression in most tissues is maintained at least until postnatal day 4. The lack of colocalization of beta-galactosidase and markers for myenteric ganglion cell types suggests that NT-3 is not expressed in these ganglia. Therefore, NT-3 expression in the GI tract is largely restricted to smooth muscle at ages when vagal axons grow into the GI tract, and when vagal mechanoreceptors form in smooth muscle, consistent with its role in these processes and in vagal sensory neuron survival.
机译：迷走胃肠（GI）传入对于调节饮食，体重和消化至关重要。但是，人们对其功能组织和发展方式知之甚少。 Neurotrophin-3（NT-3）对于迷走神经感觉神经元的生存至关重要，并在发育中的胃肠道中表达，可能有助于其生存和迷走神经传入发展的其他方面。因此，识别该外围NT-3的功能需要详细了解其在发育中的胃肠道中的表达的定位和时间。我们已经研究了从NT-3基因座表达lacZ报告基因的胚胎和新生儿，并发现NT-3主要在胃，肠和相关血管的胃肠外壁的平滑肌和胃黏膜中表达。固有层和食管上皮。在胚胎第12.5天（E12.5）时已在胃肠道的间质中检测到NT-3表达，并在E15.5时将其限制在平滑肌和固有层中，而在血管和食道上皮中的NT-3表达首次在E15.5。大多数组织中的表达至少要维持到出生后第4天。β-半乳糖苷酶和肌间神经节细胞类型标记物的共定位不足表明NT-3在这些神经节中不表达。因此，当迷走神经轴突长入胃肠道时以及迷走神经机械感受器在平滑肌中形成时，NT-3在胃肠道中的表达在很大程度上限于平滑肌，这与其在这些过程和迷走神经感觉神经元中的作用一致。

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