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首页> 外文期刊>Cell and Tissue Research >Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L.
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Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L.

机译：家蚕the内脏脂肪体组织中细胞死亡的模式。

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摘要

Cell death is a scheduled event during animal development and tissue turnover. Here, we affirm the presence of two major pathways of programmed cell death (PCD), viz. apoptotic and autophagic cell death, in the disintegrated pupal perivisceral (PV) fat body during pupal-adult metamorphosis. The acridine orange (a vital stain for apoptosis) staining pattern and DNA fragmentation assay have revealed the exact day (6th day of the pupal stage) of disintegration in the PV fat body as represented by chromatin condensation and DNA laddering. Electron microscopy and scanning electron microscopy have demonstrated the presence of cytoplasmic budding and giant autophagic vacuoles and the low numbers of mitochondria, all of which are attributes of autophagic cell death. Immunoblot analysis of proteosomal subunits 20S and 26S has established the involvement of proteolytic activity during PCD of PV tissue. Lysosomal participation during the PCD of PV tissues has been confirmed by the elevated level of the marker enzyme, acid phosphatase, which is distinct on day 6 of the pupal period. The results of the present study have thus ascertained the co-existence of both autophagic and apoptotic cell death in PV fat body tissue.
机译：细胞死亡是动物发育和组织更新期间的预定事件。在这里，我们确认存在两种主要的程序性细胞死亡（PCD）途径。 p成人变态过程中p内脏（PV）分解后脂肪体内的凋亡和自噬细胞死亡。啶橙（细胞凋亡的重要染色剂）染色模式和DNA片段化分析已揭示了PV脂肪体内崩解的确切时间（stage的第6天），以染色质浓缩和DNA标记为代表。电子显微镜和扫描电子显微镜已证明存在细胞质出芽和巨大的自噬泡以及线粒体数量少，所有这些都是自噬细胞死亡的特征。蛋白质印迹亚基20S和26S的免疫印迹分析确定了PV组织PCD期间蛋白水解活性的参与。 PV组织PCD期间的溶酶体参与已被标记酶酸性磷酸酶水平升高所证实，这在the期的第6天是明显的。因此，本研究的结果确定了PV脂肪体组织中自噬和凋亡细胞死亡的共存。

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