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首页> 外文期刊>Cellular immunology >Capture of flowing human neutrophils by immobilised immunoglobulin: Roles of Fc-receptors CD16 and CD32
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Capture of flowing human neutrophils by immobilised immunoglobulin: Roles of Fc-receptors CD16 and CD32

机译：固定的免疫球蛋白捕获流动的人类嗜中性粒细胞：Fc受体CD16和CD32的作用

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摘要

We investigated capture and activation of flowing human neutrophils through their Fc-receptors, FcR gamma IIIB (CD16) and FcR gamma IIA (CD32). Immobilised platelets bearing murine monoclonal antibody against glycoprotein IIbIIIA were able to capture and activate flowing neutrophils. The activation response was inhibited by antibody blockade of neutrophil CD32. However, capture only occurred efficiently at wall shear stress below 0.1 Pa if platelet P-selectin was blocked. If neutrophils were perfused over immobilised human IgG, many adhered at 0.025 or 0.05 Pa, but not at 0.1 Pa. Adhesion was reduced by blockade of CD16 or CD32, but blockade of CD16 had the greater effect. When neutrophils were perfused over a combination of purified P-selectin and IgG, blockade of CD 16 and CD32 inhibited activation of captured cells. Immunoglobulin deposited in tissue could capture and activate slow-flowing neutrophils. It might also potentiate inflammatory responses at higher stress if presented along with selectins. The dominant FcR for capture of neutrophils was CD 16, but with murine antibody, CD32 played a greater role. (c) 2006 Elsevier Inc. All rights reserved.
机译：我们研究了通过其Fc受体FcRγIIIB（CD16）和FcRγIIA（CD32）捕获和激活流动的人类嗜中性粒细胞。携带针对糖蛋白IIbIIIA的鼠类单克隆抗体的固定化血小板能够捕获并激活流动的嗜中性粒细胞。激活反应被中性粒细胞CD32的抗体阻断所抑制。但是，如果血小板P-选择素被阻滞，则只有在壁切应力低于0.1 Pa时才能有效地捕获。如果在固定的人IgG上灌注嗜中性粒细胞，则许多抗体会在0.025或0.05 Pa时粘附，但在0.1 Pa时不会粘附。CD16或CD32的阻断会降低粘附力，但CD16的阻断作用更大。当中性粒细胞在纯化的P-选择素和IgG的组合上灌注时，对CD 16和CD32的阻断会抑制捕获细胞的活化。沉积在组织中的免疫球蛋白可以捕获并激活缓慢流动的嗜中性粒细胞。如果与选择素一起出现，它也可能在较高压力下增强炎症反应。捕获中性粒细胞的主要FcR是CD 16，但在鼠抗体的作用下，CD32发挥了更大的作用。 （c）2006 Elsevier Inc.保留所有权利。

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