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首页> 外文期刊>Cell cycle >Small GTPase RhoA regulates cytoskeleton dynamics during porcine oocyte maturation and early embryo development
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Small GTPase RhoA regulates cytoskeleton dynamics during porcine oocyte maturation and early embryo development

机译：小GTPase RhoA调节猪卵母细胞成熟和早期胚胎发育过程中的细胞骨架动力学

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摘要

Mammalian oocyte maturation is distinguished by asymmetric division that is regulated primarily by cytoskeleton, including microtubules and microfilaments. Small Rho GTPase RhoA is a key regulator of cytoskeletal organization which regulates cell polarity, migration, and division. In this study, we investigated the roles of RhoA in mammalian oocyte meiosis and early embryo cleavage. (1) Disrupting RhoA activity or knock down the expression of RhoA caused the failure of polar body emission. This may have been due to decreased actin assembly and subsequent spindle migration defects. The involvement of RhoA in this process may have been though its regulation of actin nucleators ROCK, p-Cofilin, and ARP2 expression. (2) In addition, spindle morphology was also disrupted and p-MAPK expression decreased in RhoA inhibited or RhoA KD oocytes, which indicated that RhoA also regulated MAPK phosphorylation for spindle formation. (3) Porcine embryo development was also suppressed by inhibiting RhoA activity. Two nuclei were observed in one blastomere, and actin expression was reduced, which indicated that RhoA regulated actin-based cytokinesis of porcine embryo. Thus, our results demonstrated indispensable roles for RhoA in regulating porcine oocyte meiosis and cleavage during early embryo development.
机译：哺乳动物卵母细胞的成熟以不对称分裂为特征，该分裂主要受细胞骨架（包括微管和微丝）调控。 Small Rho GTPase RhoA是细胞骨架组织的关键调节因子，可调节细胞极性，迁移和分裂。在这项研究中，我们调查了RhoA在哺乳动物卵母细胞减数分裂和早期胚胎裂解中的作用。 （1）破坏RhoA活性或降低RhoA的表达导致极体发射失败。这可能是由于肌动蛋白装配减少和随后的主轴迁移缺陷所致。 RhoA参与此过程可能是由于其对肌动蛋白成核剂ROCK，p-Cofilin和ARP2表达的调节。 （2）此外，在RhoA抑制或RhoA KD卵母细胞中，纺锤体形态也被破坏，p-MAPK表达降低，这表明RhoA还调节MAPK磷酸化以形成纺锤体。 （3）通过抑制RhoA活性也抑制了猪胚胎发育。在一个卵裂球中观察到两个核，并且肌动蛋白的表达降低，这表明RhoA调节了猪胚胎基于肌动蛋白的胞质分裂。因此，我们的结果证明RhoA在早期胚胎发育过程中调节猪卵母细胞减数分裂和卵裂中起着不可或缺的作用。

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