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首页> 外文期刊>Rapid Communications in Mass Spectrometry: RCM >Electrospray ionization on porous spraying tips for direct sample analysis by mass spectrometry: enhanced detection sensitivity and selectivity using hydrophobic/hydrophilic materials as spraying tips
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Electrospray ionization on porous spraying tips for direct sample analysis by mass spectrometry: enhanced detection sensitivity and selectivity using hydrophobic/hydrophilic materials as spraying tips

机译：多孔喷雾头上的电喷雾电离，可通过质谱直接分析样品：使用疏水/亲水材料作为喷雾头，提高了检测灵敏度和选择性

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RATIONALE: Despite various porous materials having been widely adopted as spraying tips for direct sample analysis using electrospray ionization mass spectrometry (ESI-MS), the effect of surface property and porosity of spraying tip materials on their analytical performances is not clear. Investigation of their relationships could provide insight into the proper choice and/or design of spraying tip materials for direct sample analysis. METHODS: The effect of spraying tip materials with different polarities, including polyester and polyethylene (hydrophobic) and wood (hydrophilic), on the detection sensitivity for a variety of compounds, and on the ESI onset voltage, were studied using ESI-MS. The porosity of each type of spraying tip was characterized by scanning electron microscopy (SEM). Factors governing the detection sensitivity were determined based on the correlation of the detection sensitivity to the ESI onset voltage, the polarity, and the porosity of the spraying tip materials. RESULTS: Hydrophobic tips (i.e., polyester and polyethylene) show better detection sensitivity for polar compounds but not for non-polar compounds, while hydrophilic tips (wooden tips) show the opposite effect. This phenomenon could be due to the difference in interaction between the analytes and the tips, causing the analytes to adsorb on the tip to different extents. In addition, the micro-porous nature of the tips could facilitate solvent diffusion for transporting analytes to the tip and maintain a stable spray for recording MS data. With the proper choice of spraying tip materials, trace amount of analytes at the picomole level can be detected with minimal sample pretreatment. CONCLUSIONS: Both the polarity and the porosity of the spraying tip materials could significantly affect detection sensitivity for a wide variety of analytes. With proper choice of spraying tip material, ESI on a porous spraying tip could be a sensitive method for the direct analysis of daily life samples.
机译：理由：尽管各种多孔材料已被广泛用作使用电喷雾电离质谱（ESI-MS）进行直接样品分析的喷嘴，但不清楚喷嘴材料的表面性质和孔隙率对其分析性能的影响。通过调查它们之间的关系，可以深入了解直接进行样品分析的喷头材料的正确选择和/或设计。方法：使用ESI-MS研究了喷涂极性不同的尖端材料（包括聚酯和聚乙烯（疏水性）和木材（亲水性））对多种化合物的检测灵敏度以及ESI起始电压的影响。通过扫描电子显微镜（SEM）表征每种类型的喷嘴的孔隙率。基于检测灵敏度与ESI起始电压，极性和喷头材料的孔隙率之间的相关性，确定控制检测灵敏度的因素。结果：疏水性尖端（即聚酯和聚乙烯）对极性化合物显示出更好的检测灵敏度，但对非极性化合物则没有，而亲水性尖端（木质尖端）显示出相反的效果。这种现象可能是由于分析物和吸头之间相互作用的差异，导致分析物以不同程度吸附在吸头上。另外，针尖的微孔性质可以促进溶剂扩散，以将分析物传输到针尖，并保持稳定的喷雾以记录MS数据。通过正确选择喷头材料，可以用最少的样品预处理就可以检测到皮孔水平的痕量分析物。结论：喷嘴材料的极性和孔隙率均会显着影响多种分析物的检测灵敏度。通过正确选择喷头材料，在多孔喷头上进行ESI可能是直接分析日常生活样品的灵敏方法。

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