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首页> 外文期刊>Redox report: communications in free radical research >Hypoxia-reoxygenation enhances interleukin-8 production from U937 human monocytic cells
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Hypoxia-reoxygenation enhances interleukin-8 production from U937 human monocytic cells

机译：缺氧复氧增强U937人单核细胞产生白介素8

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摘要

Hypoxia-reoxygenation (H/R) occurs in both inflammatory spots and tumor tissues, sites in which damage is amplified either acutely or chronically through the infiltration of inflammatory cells. Interleukin-8 (IL-8) is a cytokine with chemotactic and angiogenic properties. This study was designed to investigate the effects of H/R on IL-8 production in the U937 human monocytic cell line. Two hours of hypoxia followed by 4 h of reoxygenation induced a significant increase in IL-8 protein production and IL-8 mRNA expression in U937 cells. Pretreatment with proteasome inhibitor (PSI), a peptide aldehyde known to inhibit the chymotrypsin-like activity of the 26S proteasome specifically, suppressed IL-8 protein production and IL-8 mRNA expression induced by H/R. The production of IL-8 protein induced by H/R was decreased by pioglitazone and 15-deoxy-Δ~(12,14)-PGJ_2 (15d-PGJ_2), which have been identified as peroxisome proliferator-activated receptor γ (PPAR-γ) ligands. Moreover, transfection of U937 cells with a dominant negative IκBα expression vector (IκBαM) decreased IL-8 protein production induced by H/R. These results suggest that NF-B and PPAR-γ regulate H/R-stimulated IL-8 production in U937 cells.
机译：缺氧复氧（H / R）发生在炎症部位和肿瘤组织中，在这些部位中，损伤通过炎症细胞的浸润而急性或慢性地被放大。白介素8（IL-8）是具有趋化和血管生成特性的细胞因子。本研究旨在研究H / R对U937人单核细胞系中IL-8产生的影响。缺氧2小时，然后再充氧4 h，导致U937细胞中IL-8蛋白产量和IL-8 mRNA表达显着增加。用蛋白酶体抑制剂（PSI）进行预处理，一种已知可抑制26S蛋白酶体类胰凝乳蛋白酶样活性的肽醛，可抑制H / R诱导的IL-8蛋白生成和IL-8 mRNA表达。吡格列酮和15-脱氧-Δ〜（12,14）-PGJ_2（15d-PGJ_2）降低了H / R诱导的IL-8蛋白的产生，它们被确定为过氧化物酶体增殖物激活受体γ（PPAR- γ）配体。此外，用显性负IκBα表达载体（IκBαM）转染U937细胞会降低H / R诱导的IL-8蛋白产生。这些结果表明，NF-B和PPAR-γ调节U937细胞中H / R刺激的IL-8产生。

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