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首页> 外文期刊>Cell communication & adhesion >Expression of MacMARCKS restores cell adhesion to ICAM-1-coated surface
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Expression of MacMARCKS restores cell adhesion to ICAM-1-coated surface

机译：MacMARCKS的表达可恢复细胞对ICAM-1涂层表面的粘附

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To evaluate the role of MacMARCKS, a major substrate of protein kinase C, in cell adhesion: we selected a macrophage cell line, Wehi 274.1.7. Although surface expression of beta 2-integrins can be detected on these cells, they lack the phorbol ester- or chemokine-induced adhesion to ICAM-1-coated surface, an event mediated by beta 2-integrins. Concomitantly, these cells lack expression of both MacMARCKS and its homologue, MARCKS. When wild type MacMARCKS was expressed in these cells, the phorbol ester induced adhesion to ICAM-1-coated surface increased approximately 5-fold compared to Vector transfected control cells. To further investigate the potential physiological role of MacMARCKS in this adhesion event: we also tested the effect of monocyte chemotactic protein-1,and a 3-fold increase in the adhesion to ICAM-1-coated surface was observed with MacMARCKS-transfected cells. Therefore, these data suggest that MacMARCKS is an essential component in regulating cell adhesion. [References: 17]
机译：要评估MacMARCKS（蛋白激酶C的主要底物）在细胞粘附中的作用：我们选择了巨噬细胞系Wehi 274.1.7。尽管可以在这些细胞上检测到β2-整合素的表面表达，但它们缺乏由佛波酯或趋化因子诱导的与ICAM-1包被的表面的粘附，这是由β2-整合素介导的。伴随地，这些细胞缺乏MacMARCKS及其同源物MARCKS的表达。当在这些细胞中表达野生型MacMARCKS时，与载体转染的对照细胞相比，佛波酯诱导的与ICAM-1包被的表面的粘附增加了约5倍。为了进一步研究MacMARCKS在这种粘附事件中的潜在生理作用：我们还测试了单核细胞趋化蛋白1的作用，并且用MacMARCKS转染的细胞观察到对ICAM-1涂层表面的粘附增加了3倍。因此，这些数据表明MacMARCKS是调节细胞粘附的重要组成部分。 [参考：17]

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