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首页> 外文期刊>Research communications in molecular pathology and pharmacology >Determination of a new naphthoquinone derivative, NQ12, in human plasma and urine by high-performance liquid chromatography.
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Determination of a new naphthoquinone derivative, NQ12, in human plasma and urine by high-performance liquid chromatography.

机译：高效液相色谱法测定人血浆和尿液中新的萘醌衍生物NQ12。

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A high-performance liquid chromatographic method was developed for the determination of a new phospholipase A2 inhibitor, NQ12, in human plasma and urine. The sample preparation was simple: 2 volumes of acetonitrile were added to the biological samples to deproteinize it. A 50-microl aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was 0.05M acetate buffer (pH 3) : acetonitrile : methanol (30:45:25, v/v/v) and run at a flow rate of 1.5 ml/min. The column effluent was monitored by a UV detector set at 298 nm. The retention times for NQ12 and the internal standard were approximately 5.5 and 7.0 min, respectively. The detection limits for NQ12 in human plasma and urine were all 20 ng/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 11.8%) for human plasma and urine. No interferences from endogenous substances were found.
机译：开发了一种高效液相色谱方法，用于测定人血浆和尿液中的新型磷脂酶A2抑制剂NQ12。样品制备很简单：将2体积的乙腈添加到生物样品中以使其脱蛋白。将50微升等分试样的上清液注入到C18反相柱上。所使用的流动相为0.05M乙酸盐缓冲液（pH 3）：乙腈：甲醇（30:45:25，v / v / v），以1.5 ml / min的流速运行。通过设置在298 nm的UV检测器监控柱流出物。 NQ12和内标的保留时间分别约为5.5分钟和7.0分钟。人血浆和尿液中NQ12的检出限均为20 ng / ml。对于人血浆和尿液，该测定的变异系数（日内和日间）通常较低（低于11.8％）。没有发现来自内源性物质的干扰。

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