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首页> 外文期刊>Research communications in molecular pathology and pharmacology >Fas/Fas-ligand expressions in peripheral-blood mononuclear cells of patients with myelodysplastic syndromes.
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Fas/Fas-ligand expressions in peripheral-blood mononuclear cells of patients with myelodysplastic syndromes.

机译：骨髓增生异常综合征患者外周血单个核细胞中Fas / Fas-配体的表达。

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Increased expressions of Fas and Fas-ligand (Fas-L) in bone marrow cells of myelodysplastic syndromes (MDS) have been reported, and large number of these "cell-death signals" might explain molecular basis for exacerbation of apoptosis in marrow cells of these syndromes. However, expression of these molecules or progression of apoptosis in peripheral-blood mononuclear cells (PBMCs) in MDS has little been investigated. In the present study, we compared expression of these cell-death molecules and percentages of apoptotic cells in PBMCs between MDS patients and healthy subjects. PBMCs were obtained from 7 MDS patients and 8 age-matched healthy controls. Five out of 7 patients were MDS with refractory anemia (RA) type, while the other 2 were MDS-RA with excess of blasts (MDS-RAEB) type. Percentages of PBMCs expressing Fas, Fas-L, and phosphatidylserine as a cell apoptosis-marker were determined by staining cells with FITC-labeled anti-CD95 (Fas) antibody, biotinyl anti Fas-L antibody, and annexin V, respectively. The cells were subsequently analyzed with flow cytometry. The mean (SD) percentage of Fas-expressing PBMCs in MDS group was 55.3 (13.9), whereas the value in healthy subjects was 30.6 (8.8) %, and thus the ratio of Fas positive cells in PBMCs of MDS was significantly higher than that of healthy subjects (p < 0.002). In contrast, the mean (SD) of Fas-L expressing PBMCs in MDS (n=5) was 18.4 (12.2) %, which was significantly lower (p < 0.02) than that in healthy subjects (34.4 +/- 8.1%; n=7). The mean (SD) of apoptotic PBMCs detected as annexin V-positive, non-necrotic cells in MDS (n=7) was 23.3 (7.5) %, which was not significantly different from that in healthy subjects (22.2 +/- 7.8%; n=8). Thus, PBMCs in MDS express high levels of Fas, whereas they conversely exhibit low levels of Fas-L, which may result in prevention of apoptosis by the death signals, and in cell-survival in these cells.
机译：据报道，骨髓增生异常综合征（MDS）骨髓细胞中Fas和Fas-配体（Fas-L）的表达增加，这些“细胞死亡信号”的大量可能解释了加重骨髓细胞凋亡的分子基础。这些综合症。但是，很少研究这些分子在MDS中的表达或在外周血单核细胞（PBMC）中的凋亡进程。在本研究中，我们比较了MDS患者和健康受试者在PBMC中这些细胞死亡分子的表达和凋亡细胞的百分比。从7名MDS患者和8名年龄匹配的健康对照中获得PBMC。 7例患者中有5例是MDS难治性贫血（RA）型，而其他2例是MDS-RA伴有原始细胞过多（MDS-RAEB）型。通过分别用FITC标记的抗CD95（Fas）抗体，生物素抗Fas-L抗体和Annexin V染色细胞来确定表达Fas，Fas-L和磷脂酰丝氨酸作为细胞凋亡标记物的PBMC的百分比。随后用流式细胞仪分析细胞。 MDS组中表达Fas的PBMC的平均（SD）百分比为55.3（13.9），而健康受试者的平均值为30.6（8.8）％，因此MDS的PBMC中Fas阳性细胞的比例显着高于健康受试者（p <0.002）。相反，MDS（n = 5）中表达Fas-L的PBMC的平均值（SD）为18.4（12.2）％，显着低于健康受试者（34.4 +/- 8.1％）的（p <0.02）; n = 7）。在MDS（n = 7）中被检测为膜联蛋白V阳性，非坏死细胞的凋亡PBMC的平均值（SD）为23.3（7.5）％，与健康受试者的平均值（22.2 +/- 7.8％）并无显着差异。 ; n = 8）。因此，MDS中的PBMC表达高水平的Fas，而相反地它们显示低水平的Fas-L，这可能导致通过死亡信号阻止凋亡，并导致这些细胞的细胞存活。

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