...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Research in Veterinary Science >Adenosine deaminase activity in serum, erythrocytes and lymphocytes of rats infected with Leptospira icterohaemorrhagiae.
【24h】

Adenosine deaminase activity in serum, erythrocytes and lymphocytes of rats infected with Leptospira icterohaemorrhagiae.

机译：感染了黄腐螺旋体出血性出血热的大鼠的血清，红细胞和淋巴细胞中的腺苷脱氨酶活性。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Leptospirosis is a systemic disease of humans and domestic animals, mainly dogs, cattle and swine. The course of human leptospirosis varies from mild to severe fatal forms and the most severe form of human leptospirosis is principally caused by Leptospira interrogans serovar icterohaemorrhagiae (L. icterohaemorrhagiae). The enzyme adenosine deaminase (ADA) plays an important role in the production and differentiation of blood cells. The aim of this study was to evaluate the activity of ADA in serum, erythrocytes and lymphocytes of rats infected with L. icterohaemorrhagiae, as compared with non-infected rats. Twenty-four adult rats, divided into two uniform groups (A and B) were used for the enzymatic assays. The animals in Group B were inoculated intraperitoneally with 2x108 leptospires/rat, and the rodents in Group A (control) were not-inoculated. Blood collection was performed on days 5 and 15 post-infection (PI) and the blood used to assess the ADA activity. The infection by L. icterohaemorrhagiae altered erythrocyte count, hemoglobin concentration and hematocrit, causing a decrease in all these parameters on day 15 PI. Lymphocytes decreased significantly on day 15 PI, and ADA activity in serum was inhibited in infected rats on days 5 and 15 PI and its activity in erythrocytes were increased on day 5 PI. On day 5 PI, we found an increase in ADA activity in erythrocytes of infected rats. No correlation was observed between hematocrit and erythrocyte ADA activity on days 5 and 15 PI. The ADA activity was inhibited in rats infected on day 15 PI. A positive correlation (r2=60) was also observed between the number of lymphocytes and ADA activity in lymphocytes on day 15 PI (P<0.05). In conclusion, our results showed that the ADA activity is altered in serum, lymphocytes and erythrocytes in experimental infection by L. icterohaemorrhagiae in rats, concomitantly with hematological parameters.
机译：钩端螺旋体病是人和家畜（主要是狗，牛和猪）的全身性疾病。人类钩端螺旋体病的病程从轻度到严重的致命形式不等，人类钩端螺旋体病的最严重形式主要是由问号钩端螺旋体血清型黄疸出血性出血病（ L。icterohaemorrhagiae < / i>）。腺苷脱氨酶（ADA）在血细胞的产生和分化中起重要作用。这项研究的目的是评估ADA在被iL感染的大鼠的血清，红细胞和淋巴细胞中的活性。黄疸出血，与未感染的大鼠相比。将二十四只成年大鼠分为两个统一的组（A和B）用于酶法测定。 B组动物腹膜内接种2x10 8 钩端螺旋体/大鼠，A组（对照组）啮齿动物未接种。在感染后第5天和第15天进行采血，并将血液用于评估ADA活性。 L感染。黄疸出血改变了红细胞计数，血红蛋白浓度和血细胞比容，导致在PI第15天所有这些参数均下降。在感染后第15天，淋巴细胞显着减少，在感染后第5天和第15天，感染大鼠的血清ADA活性受到抑制，在感染第5天，其红细胞活性增加。在第5天，我们发现被感染大鼠的红细胞中ADA活性增加。在感染后第5天和第15天，血细胞比容和红细胞ADA活性之间没有相关性。在PI第15天感染的大鼠中ADA活性受到抑制。 PI第15天时，淋巴细胞数量与淋巴细胞中ADA活性之间也存在正相关（ r 2 = 60）（ P < 0.05）。总而言之，我们的结果表明，在实验性感染被 L感染后，血清，淋巴细胞和红细胞中的ADA活性发生了改变。大鼠黄疸出血，并伴有血液学参数。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号