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首页> 外文期刊>Research in Veterinary Science >Local and systemic immune responses in mice to intranasal delivery of peptides representing bovine respiratory syncytial virus epitopes encapsulated in poly (DL-lactide-co-glycolide) microparticles
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Local and systemic immune responses in mice to intranasal delivery of peptides representing bovine respiratory syncytial virus epitopes encapsulated in poly (DL-lactide-co-glycolide) microparticles

机译：小鼠对鼻内递送代表包裹在聚（DL-丙交酯-共-乙交酯）微粒中的牛呼吸道合胞病毒抗原决定簇的肽的局部和全身免疫应答

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摘要

The potential of a microparticulate vaccine delivery system in eliciting a specific mucosal antibody response in the respiratory tract of mice was evaluated. Two vaccine candidate peptides representing epitopes from the G attachment and F fusion antigens from bovine respiratory syncytial virus (BRSV) were encapsulated into poly(DL- lactide co-glycolide) biodegradable microparticles. The encapsulation process did not denature the entrapped peptides as verified by detection of peptide-specific antibodies in mucosal secretions by ELISA using peptide as antigen. Following intranasal immunisation, the encapsulated peptides induced stronger upper and lower respiratory tract specific-IgA responses, respectively, than the soluble peptide forms. Moreover, a strong peptide-specific cell-mediated immune response was measured in splenocytes in vitro from the mice inoculated with the encapsulated peptides compared to their soluble form alone indicating that migration of primed T cells had taken place from the site of mucosal stimulation in the upper respiratory tract to the spleen. These results act as a foundation for vaccine efficacy studies in large animal BRSV challenge models
机译：评价了微粒疫苗递送系统在小鼠呼吸道中引起特异性粘膜抗体应答的潜力。将代表来自G附着的表位和来自牛呼吸道合胞病毒（BRSV）的F融合抗原的两个疫苗候选肽封装到聚（DL-丙交酯-乙交酯）可生物降解的微粒中。通过使用肽作为抗原的ELISA检测粘膜分泌物中的肽特异性抗体，证实了包封过程没有使捕获的肽变性。鼻内免疫后，与可溶性肽形式相比，包封的肽分别诱导更强的上呼吸道和下呼吸道特异性IgA反应。此外，与单独的可溶形式相比，在用封装的肽接种的小鼠的脾脏细胞中，在体外脾细胞中测量到了强的肽特异性细胞介导的免疫反应，表明引发的T细胞已从粘膜刺激部位迁移。上呼吸道至脾脏。这些结果为大型动物BRSV攻击模型中疫苗功效研究奠定了基础

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