• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell communication & adhesion >The Voltage Gates of Connexin Channels are Sensitive to CO_2
【24h】

The Voltage Gates of Connexin Channels are Sensitive to CO_2

机译：连接蛋白通道的电压门对CO_2敏感

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cx45 channel sensitivity to CO_2, transjunctional voltage (V_j) and inhibition of calmodulin (CaM) expression was tested in oocytes by dual voltage-clamp. Cx45 channels are very sensitive to V_j and close preferentially by the slow gate, likely the same as the chemical gate. With CO_2-induced drop in junctional conductance (G_j), the speed of V_j-dependent inactivation of junctional current (I_j) and V_j sensitivity increased. With 40 mV V_j, the τ of single exponential I_j decay reversibly decreased by ～40% with CO_2, and G_(j steady state)/G_(j peak) decreased multiphasically, indicating that kinetics and V_j sensitivity of chemical/slow-V_j gating are altered by changes in [H~+]_i and/or [Ca~(2+)]_i. With 15 min exposure to CO_2, G_j dropped to 0% in controls and by ～17% following CaM expression inhibition; similarly, V_j sensitivity decreased significantly. This indicates that the speed and sensitivity of V_j-dependent inactivation of Cx45 channels are increased by CO_2, and that CaM plays a role in gating. Cx32 channels behaved similarly, but the drop in both G_(j steady state)/G_(j peak) and τ with CO_2 matched more closely that of G_(j peak). In contrast, sensitivity and speed of V_j gating of Cx40 and Cx26 channels decreased, rather than increased, with CO_2 application.
机译：通过双电压钳测试了卵母细胞中Cx45通道对CO_2的敏感性，跨结电压（V_j）和钙调蛋白（CaM）表达抑制。 Cx45通道对V_j非常敏感，并优先通过慢速门关闭，可能与化学门相同。随着CO_2诱导的结电导（G_j）下降，依赖于V_j的结电流（I_j）失活的速度和V_j的灵敏度增加。 V_j为40 mV时，CO_2时单指数I_j衰减的τ可逆地降低约40％，G_（j稳态）/ G_（j峰值）呈多相下降，表明化学/慢V_j门控的动力学和V_j敏感性通过[H〜+] _ i和/或[Ca〜（2 +）] _ i的更改来更改。暴露于CO_2 15分钟后，对照中的G_j降至0％，CaM表达抑制后G_j降低约-17％。同样，V_j灵敏度显着下降。这表明CO_2增加了Vxj依赖的Cx45通道失活的速度和灵敏度，并且CaM在门控中起作用。 Cx32通道的行为类似，但是G_（j稳态）/ G_（j峰值）和τ随CO_2的下降更接近G_（j峰值）。相反，随着CO_2的应用，Cx40和Cx26通道的V_j门控的灵敏度和速度降低而不是提高。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号