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首页> 外文期刊>Regulatory peptides. >Primary structure of solitary form of gonadotropin-releasing hormone (GnRH) in cichlid pituitary; three forms of GnRH in brain of cichlid and pumpkinseed fish.
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Primary structure of solitary form of gonadotropin-releasing hormone (GnRH) in cichlid pituitary; three forms of GnRH in brain of cichlid and pumpkinseed fish.

机译：丽鱼科鱼垂体中促性腺激素释放激素（GnRH）的单独形式的一级结构;丽鱼科鱼和南瓜子鱼脑中三种形式的GnRH。

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摘要

GnRH is a decapeptide family with at least nine distinct structures. Vertebrates, except for most placental mammals, have more than one of these GnRH forms within the brain. We report chromatographical and immunological evidence that three forms of GnRH are in the brains of both cichlid (Haplochromis burtoni) and pumpkinseed (Lepomis gibbosus) fishes. We argue that the three forms correspond to those previously described as sea bream GnRH (sbGnRH), chicken GnRH-II and salmon GnRH. In contrast, only one GnRH form was present in the pituitary of the cichlid and is identified as sbGnRH by amino acid sequence. This is the first report in which the primary structure of GnRH is determined from pituitary tissue. The N-terminus was identified by monitoring the digestion of the peptide by pyroglutamate aminopeptidase with matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The amidation of the C-terminus was established using an esterification procedure for monitoring with MALDI-MS. Thisreport supports the idea that three forms of GnRH within one species is widespread in the order Perciformes. The present study establishes sbGnRH as the third GnRH form in H. burtoni and predicts that sbGnRH is synthesized in preoptic neurons, then transported to the pituitary in the preoptic-hypophyseal axons for the release of one or both gonadotropins.
机译：GnRH是具有至少9个不同结构的十肽家族。除大多数胎盘哺乳动物外，脊椎动物的大脑中GnRH形式不止一种。我们报告色谱和免疫学证据，三种形式的GnRH是在丽鱼科鱼（Haplochromis burtoni）和南瓜子鱼（Lepomis gibbosus）的大脑中。我们认为这三种形式对应于先前描述的鲷鱼GnRH（sbGnRH），鸡肉GnRH-II和鲑鱼GnRH。相反，在丽鱼科鱼的垂体中仅存在一种GnRH形式，并且通过氨基酸序列被鉴定为sbGnRH。这是第一份从垂体组织确定GnRH一级结构的报告。通过用基质辅助激光解吸/电离（MALDI）质谱（MS）监测焦谷氨酸氨基肽酶对肽的消化来鉴定N末端。使用酯化程序建立C末端的酰胺化状态，以便用MALDI-MS进行监测。该报告支持以下观点：一个物种内三种形式的GnRH以Perciformes顺序分布。本研究建立了sbGnRH作为伯氏嗜血杆菌的第三种GnRH形式，并预测sbGnRH在视前神经元中合成，然后转运至垂体前垂体轴突下垂体中释放一种或两种促性腺激素。

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