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首页> 外文期刊>Cell cycle >Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes.
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Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes.

机译：Spc25表达的扰动影响小鼠卵母细胞的减数分裂纺锤体组织，染色体排列和纺锤体装配检查点。

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摘要

Spc25 is a component of the Ndc80 complex which consists of Ndc80, Nuf2, Spc24, and Spc25. Previous work has shown that Spc25 is involved in regulation of kinetochore microtubule attachment and the spindle assembly checkpoint in mitosis. The roles of Spc25 in meiosis remain unknown. Here, we report its expression, localization and functions in mouse oocyte meiosis. The Spc25 mRNA level gradually increased from the GV to MI stage, but decreased by MII during mouse oocyte meiotic maturation. Immunofluorescent staining showed that Spc25 was restricted to the germinal vesicle, and associated with chromosomes during all stages after GVBD. Overexpression of Spc25 by mRNA injection resulted in oocyte meiotic arrest, chromosome misalignment and spindle disruption. Conversely, Spc25 RNAi by siRNA injection resulted in precocious polar body extrusion and caused severe chromosome misalignment and aberrant spindle formation. Our data suggest that Spc25 is required for chromosome alignment, spindle formation, and proper spindle checkpoint signaling during meiosis.
机译：Spc25是Ndc80复合体的一个组件，该复合体由Ndc80，Nuf2，Spc24和Spc25组成。以前的工作表明，Spc25参与调节有丝线粒的微管附着和纺锤体装配检查点。 Spc25在减数分裂中的作用仍然未知。在这里，我们报告其在小鼠卵母细胞减数分裂中的表达，定位和功能。 Spc25 mRNA水平从GV到MI期逐渐升高，但在小鼠卵母细胞减数分裂成熟期间被MII降低。免疫荧光染色显示，SPC25仅限于发芽囊泡，并且在GVBD后的所有阶段均与染色体相关。 mRNA注射使Spc25过度表达导致卵母细胞减数分裂停滞，染色体错位和纺锤体破坏。相反，通过siRNA注射的Spc25 RNAi导致性极体早熟挤出，并导致严重的染色体错位和异常纺锤体形成。我们的数据表明，Spc25是减数分裂过程中染色体对齐，纺锤体形成和正确的纺锤体检查点信号传递所必需的。

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