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首页> 外文期刊>Radiotherapy and oncology: Journal of the European Society for Therapeutic Radiology and Oncology >Genetic determination of chromosomal radiosensitivities in G0- and G2-phase human lymphocytes.
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Genetic determination of chromosomal radiosensitivities in G0- and G2-phase human lymphocytes.

机译：G0和G2期人类淋巴细胞中染色体放射敏感性的遗传测定。

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BACKGROUND AND PURPOSE: The radiosensitivity of human lymphocytes measured using a G0- or G2-assay has been linked with an individual's risk of developing normal tissue complications following radiotherapy. This study was performed to increase basic knowledge of the genetics of the human radiation response, and chromosomal aberration induction in particular. MATERIALS AND METHODS: The study was carried out with blood samples taken from 15 monozygotic twin pairs. G0-assay was performed for cells irradiated with 6 Gy counting only deletions and G2-assay for cells irradiated with 0.5 Gy scoring only chromatid breaks. RESULTS: The mean number of deletions measured at 6 Gy for all 30 samples using the G0-assay amounted to 2.96+/-0.37 (means+/-SD), which corresponds to a coefficient of variation (CV) of 13%. There is a highly significant intra-pair correlation for this number among twins (r(2)=0.911) demonstrating that this parameter is mostly determined by genetic factors. According to the mean number of deletions, a theoretical classification based on the definition < or = MV-SD as resistant, MV+/-SD as normal and > or = MV+SD as sensitive was made, identifying two pairs as sensitive or resistant, respectively, while nine were normal and two pairs are intermediate. For chromatid breaks measured at 0.5 Gy with the G2-assay the mean number was 1.35+/-0.42 (means+/-SD) corresponding to a CV of 31%. There was again a strong intra-pair correlation among twins with r(2)=0.837 showing that this sensitivity is also determined mostly by genetic factors. There was, however, no inter-assay correlation between the G0- and G2-sensitivity (r(2)=0.006) demonstrating that these two sensitivities depend on different genetic factors. CONCLUSION: The chromosomal radiosensitivity of lymphocytes as defined by G0- or G2-assay is largely determined by different genetic factors, which may allow the use of genetic profiling as an indicator of the respective individual radiosensitivity.
机译：背景与目的：使用G0或G2分析法测量的人类淋巴细胞放射敏感性与个体放疗后发生正常组织并发症的风险有关。进行这项研究的目的是增加人类辐射反应的遗传学基础知识，尤其是染色体畸变诱导的基础知识。材料与方法：本研究是从15对单卵双生子对中抽取的血样进行的。对仅用缺失计数的6 Gy照射的细胞进行G0分析，而对仅用染色单体断裂评分的0.5 Gy照射的细胞进行G2分析。结果：使用G0分析在所有30个样品中，在6 Gy处测得的平均缺失数为2.96 +/- 0.37（平均值+/- SD），对应于13％的变异系数（CV）。在双胞胎中，这个数字具有很高的配对对内相关性（r（2）= 0.911），表明该参数主要由遗传因素决定。根据平均缺失数，基于以下定义进行理论分类：MV-SD为抗药性，MV +/- SD为正常，而MV + SD为敏感，定义两对为敏感或抗药性，分别是正常的九对和中间的两对。对于使用G2分析在0.5 Gy下测得的染色单体断裂，平均数为1.35 +/- 0.42（平均值+/- SD），对应CV为31％。 r（2）= 0.837的双胞胎之间再次存在强烈的配对对内相关性，表明这种敏感性也主要由遗传因素决定。但是，G0和G2敏感性之间没有分析间相关性（r（2）= 0.006），表明这两种敏感性取决于不同的遗传因素。结论：G0-或G2-测定所定义的淋巴细胞的染色体放射敏感性在很大程度上取决于不同的遗传因素，这可能允许使用基因谱作为各自个体放射敏感性的指标。

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