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首页> 外文期刊>Radiotherapy and oncology: Journal of the European Society for Therapeutic Radiology and Oncology >Susceptible genes and molecular pathways related to heavy ion irradiation in oral squamous cell carcinoma cells.
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Susceptible genes and molecular pathways related to heavy ion irradiation in oral squamous cell carcinoma cells.

机译：与口腔鳞状细胞癌细胞中重离子辐射有关的易感基因和分子途径。

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BACKGROUND AND PURPOSE: Heavy ion beams are high linear energy transfer (LET) radiation characterized by a higher relative biologic effectiveness than low LET radiation. The aim of the current study was to determine the difference of gene expression between heavy ion beams and X-rays in oral squamous cell carcinoma (OSCC)-derived cells. MATERIALS AND METHODS: The OSCC cells were irradiated with accelerated carbon or neon ion irradiation or X-rays using three different doses. We sought to identify genes the expression of which is affected by carbon and neon ion irradiation using Affymetrix GeneChip analysis. The identified genes were analyzed using the Ingenuity Pathway Analysis Tool to investigate the functional network and gene ontology. Changes in mRNA expression in the genes were assessed by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). RESULTS: The microarray analysis identified 84 genes that were modulated by carbon and neon ion irradiation at all doses in OSCC cells. Among the genes, three genes (TGFBR2, SMURF2, and BMP7) and two genes (CCND1 and E2F3), respectively, were found to be involved in the transforming growth factor beta-signaling pathway and cell cycle:G1/S checkpoint regulation pathway. The qRT-PCR data from the five genes after heavy ion irradiation were consistent with the microarray data (P < 0.01). CONCLUSION: Our findings should serve as a basis for global characterization of radiation-regulated genes and pathways in heavy ion-irradiated OSCC.
机译：背景和目的：重离子束是高线性能量转移（LET）辐射，其特征在于，其相对生物有效性高于低LET辐射。本研究的目的是确定口腔鳞状细胞癌（OSCC）源性细胞中重离子束和X射线之间基因表达的差异。材料与方法：用三种不同剂量的加速碳或氖离子辐照或X射线辐照OSCC细胞。我们寻求使用Affymetrix GeneChip分析来鉴定其表达受碳和氖离子辐射影响的基因。使用Ingenuity Pathway分析工具分析已鉴定的基因，以研究功能网络和基因本体。通过实时定量逆转录酶-聚合酶链反应（qRT-PCR）评估基因中mRNA表达的变化。结果：微阵列分析确定了OSCC细胞中所有剂量的碳和氖离子辐射调节的84个基因。在这些基因中，发现三个基因（TGFBR2，SMURF2和BMP7）和两个基因（CCND1和E2F3）分别与转化生长因子β信号通路和细胞周期：G1 / S检查点调节通路有关。重离子辐照后来自五个基因的qRT-PCR数据与微阵列数据一致（P <0.01）。结论：我们的发现应作为整体表征重离子辐照OSCC中辐射调节基因和途径的基础。

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