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首页> 外文期刊>Cellular Physiology and Biochemistry >Inhibitory Effect of Thymol on Suicidal Erythrocyte Death
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Inhibitory Effect of Thymol on Suicidal Erythrocyte Death

机译：百里香酚对自杀性红细胞死亡的抑制作用

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摘要

The antibacterial plant component thymol has antioxidant activity. Oxidative stress is known to activate Ca2+-permeable cation channels with subsequent Ca2+ entry, activation of Ca2+-sensitive K+ channels, cell shrinkage, cell membrane scrambling and phosphatidylserine exposure at the erythrocyte surface. Cell shrinkage and phosphatidylserine exposure are typical features of suicidal erythrocyte death or eryptosis. Eryptotic cells are cleared from circulating blood thus causing anemia and may adhere to the vascular wall thus interfering with the microcirculation. The present experiments explored whether thymol interacts with eryptosis. Annexin V-binding was utilized to determine phosphatidylserine exposure, forward scatter to detect alterations of cell volume and Fluo3 fluorescence to depict changes of the cytosolic Ca2+ activity. Oxidative stress (30 min. 0.3 mM tert-butylhydroperoxide), energy depletion (48 h glucose removal) and isotonic cell shrinkage (48 h replacement of extracellular Cl- with gluconate) significantly increased annexin V-binding and decreased the forward scatter, effects significantly blunted in the presence of thymol 2.5-20 mu g/ml. Thymol is a potent inhibitor of suicidal erythrocyte death particularly following oxidative stress. In conclusion, thymol may be useful incounteracting anemia and impairment of microcirculation.
机译：抗菌植物成分百里酚具有抗氧化活性。众所周知，氧化应激会激活Ca2 +渗透性阳离子通道，随后进入Ca2 +通道，激活Ca2 +敏感的K +通道，细胞收缩，细胞膜争夺以及磷脂酰丝氨酸在红细胞表面的暴露。细胞收缩和磷脂酰丝氨酸暴露是自杀性红细胞死亡或隐匿的典型特征。血液循环中清除了隐性细胞，从而引起贫血，并可能粘附在血管壁上，从而干扰了微循环。本实验探讨了百里酚是否与隐伏相互作用。利用膜联蛋白V-结合来确定磷脂酰丝氨酸的暴露，正向散射以检测细胞体积的变化，并通过Fluo3荧光来描述胞浆Ca2 +活性的变化。氧化应激（30分钟，0.3 mM氢过氧化叔丁基），能量耗竭（去除葡萄糖48小时）和等渗细胞收缩（用葡萄糖酸盐替代细胞外Cl- 48小时）显着增加了膜联蛋白V结合并降低了前向散射，效果显着在百里酚2.5-20μg/ ml存在下变钝。百里酚是有效的自杀性红细胞死亡抑制剂，尤其是在氧化应激后。总之，百里香酚可能用于抵抗贫血和微循环障碍。

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