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首页> 外文期刊>Cell cycle >Human umbilical cord blood-derived stromal cells: Multifaceted regulators of megakaryocytopoiesis.
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Human umbilical cord blood-derived stromal cells: Multifaceted regulators of megakaryocytopoiesis.

机译：人脐带血来源的基质细胞：巨核细胞生成的多方面调节者。

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It has been demonstrated that stromal cell precursors exist in human umbilical cord blood. After being cultured in vitro, these cells are called human umbilical cord blood-derived stromal cells (hUCBDSCs). However, the role of hUCBDSCs in hematopoiesis is still unclear. We have previously shown that hUCBDSCs are superior to human bone marrow stromal cells (hBMSCs) at enhancing the expansion of megakaryocyte colony forming units (CFU-Meg). Based on this observation, we postulated that hUCBDSCs might promote megakaryocytopoiesis. To test this hypothesis, we developed a megakaryocyte/hUCBDSC co-culture model and a hematopoietic microenvironment injury model in nude mice. We explored the ability and mechanisms by which hUCBDSCs promoted the proliferation of megakaryocytes in vitro, and we also explored their capacity to restore the hematopoietic microenvironment in vivo. As expected, hUCBDSCs were more effective than hBMSCs at enhancing the proliferation of megakaryocyte lines from HEL cells and restoring megakaryocytopoiesis in a hematopoietic microenvironment injury model in nude mice. Thrombopoietin (TPO) and stromal cell derived factor-1 (SDF-1) are two of the key factors underlying this capacity. We also found that gap junction intercellular communication (GJIC) between HEL cells and hUCBDSCs might be partially absent. Our data provide the first evidence that hUCBDSCs play a regulatory role during megakaryocytopoiesis, which might be important for designing treatments for patients with megakaryocytic injury.
机译：已经证明人脐带血中存在基质细胞前体。在体外培养后，这些细胞称为人脐带血来源的基质细胞（hUCBDSCs）。但是，hUCBDSCs在造血中的作用仍不清楚。先前我们已经证明，hUCBDSCs在增强巨核细胞集落形成单位（CFU-Meg）的扩增方面优于人骨髓基质细胞（hBMSCs）。基于此观察，我们推测hUCBDSCs可能促进巨核细胞生成。为了验证该假设，我们在裸鼠中建立了巨核细胞/ hUCBDSC共培养模型和造血微环境损伤模型。我们探讨了hUCBDSCs在体外促进巨核细胞增殖的能力和机制，还探讨了其在体内恢复造血微环境的能力。不出所料，在裸鼠造血微环境损伤模型中，hUCBDSC比hBMSC更有效地增强了HEL细胞中巨核细胞系的增殖并恢复了巨核细胞生成。血小板生成素（TPO）和基质细胞衍生因子1（SDF-1）是构成这种能力的两个关键因素。我们还发现，HEL细胞和hUCBDSCs之间的间隙连接细胞间通讯（GJIC）可能部分不存在。我们的数据提供了第一个证据，证明hUCBDSCs在巨核细胞生成过程中起调节作用，这对于设计巨核细胞损伤患者的治疗方法可能很重要。

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