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首页> 外文期刊>Cell cycle >Molecular cloning and characterization of mitogen-activated protein kinase 2 in Trypanosoma cruzi.
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Molecular cloning and characterization of mitogen-activated protein kinase 2 in Trypanosoma cruzi.

机译：克鲁氏锥虫中有丝分裂原活化蛋白激酶2的分子克隆和表征。

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Mitogen-activated protein kinase (MAPK) pathways are major signal transduction systems by which eukaryotic cells convert environmental cues to intracellular events such as proliferation and differentiation. We have identified a Trypanosoma cruzi homologue of the MAPK family that we have called TcMAPK2. Sequence analyses demonstrates TcMAPK2 has high homology with lower eukaryotic ERK2 but has significant differences from mammalian ERK2. Enzymatic assays of both recombinant TcMAPK2 and native protein obtained by immunoprecipitation using anti-TcMAPK2 demonstrated that both preparations of TcMAPK2 were catalytically active. Immunofluorescence analysis of the subcellular localization of TcMAPK2 determined it is mainly cytoplasmic in epimastigotes, along the flagella in trypomastigotes and on the plasma membrane of intracellular amastigotes. Phosphorylated TcMAPK2 was highest in trypomastigotes and lowest in amastigotes. Recombinant TcMAPK2 was able to phosphorylate the recombinant protein of a cAMP specific phosphodiesterase. Overexpression of TcMAPK2 in epimastigotes inhibited growth and development leading to death. TcMAPK2 has an important role in the stress response of the parasite and may be important in regulating proliferation and differentiation.
机译：丝裂原激活的蛋白激酶（MAPK）途径是主要的信号转导系统，真核细胞通过该途径将环境线索转换为细胞内事件，例如增殖和分化。我们已经鉴定了我们称为TcMAPK2的MAPK家族的锥虫锥虫同源物。序列分析表明，TcMAPK2与较低的真核ERK2具有高度同源性，但与哺乳动物ERK2有显着差异。通过使用抗TcMAPK2进行免疫沉淀获得的重组TcMAPK2和天然蛋白的酶促测定表明，两种TcMAPK2制剂均具有催化活性。对TcMAPK2的亚细胞定位进行的免疫荧光分析确定，它主要在副鞭毛虫，锥毛鞭毛虫的鞭毛和胞内变形虫的质膜上呈胞质。磷酸化的TcMAPK2在锥虫中最高，而在变形虫中最低。重组TcMAPK2能够磷酸化cAMP特异性磷酸二酯酶的重组蛋白。 TmMAPK2在副鞭毛动物中的过表达抑制生长发育，导致死亡。 TcMAPK2在寄生虫的应激反应中起重要作用，并且可能在调节增殖和分化中起重要作用。

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