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首页> 外文期刊>Cellular Physiology and Biochemistry >A new role for aquaporin 7 in insulin secretion
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A new role for aquaporin 7 in insulin secretion

机译：水通道蛋白7在胰岛素分泌中的新作用

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Several insulinotropic agents were recently reported to cause β-cell swelling. The possible participation of AQP7 to water transport was investigated in AQP7 ~(+/ +) or AQP7 ~(-/-) mice. Methods: Aquaporin expression, insulin secretion, cell swelling and electrical activity were investigated in pancreatic islets. Results: RT-PCR revealed the expression of AQP5 and AQP8 mRNA. Double immunofluorescent labeling indicated their presence in β-cells. Whilst basal insulin release from isolated pancreatic islets incubated at 2.8 mM D-glucose did not differ between AQP7 ~(+/ +) or AQP7 ~(-/-) mice, the secretion of insulin evoked by the omission of 50 mM NaCl, the substitution of 50 mM NaCl by 100 mM glycerol or a rise in D-glucose concentration to 8.3 mM and 16.7 mM was severely impaired in the islets from AQP7 ~(-/-) mice. Yet, exposure of β-cells to either the hypotonic medium or a rise in D-glucose concentration caused a similar degree of swelling and comparable pattern of electrical activity in cells from AQP7 ~(+/ +) and AQP7 ~(-/-) mice. Both the cell swelling and change in membrane potential were only impaired in AQP7 ~(-/-) cells when exposed to 50 mM glycerol. Conclusion: It is proposed, therefore, that AQP7 may, directly or indirectly, play a role at a distal site in the exocytotic pathway.
机译：最近报道了几种促胰岛素剂引起β细胞肿胀。在AQP7〜（+ / +）或AQP7〜（-/-）小鼠中研究了AQP7可能参与水运输。方法：研究胰岛中水通道蛋白的表达，胰岛素分泌，细胞肿胀和电活动。结果：RT-PCR显示AQP5和AQP8 mRNA的表达。双重免疫荧光标记表明它们存在于β细胞中。尽管AQP7〜（+ / +）或AQP7〜（-/-）小鼠在2.8 mM D-葡萄糖温育下从分离的胰岛中释放基础胰岛素没有差异，但由于遗漏了50 mM NaCl而引起了胰岛素的分泌。在来自AQP7〜（-/-）小鼠的胰岛中，严重损害了用100 mM甘油代替50 mM NaCl或D-葡萄糖浓度升高至8.3 mM和16.7 mM的情况。然而，β细胞暴露于低渗介质或D-葡萄糖浓度升高导致AQP7〜（+ / +）和AQP7〜（-/-）产生相似程度的肿胀和类似的电活动模式老鼠。当暴露于50 mM甘油中时，仅在AQP7〜（-/-）细胞中细胞肿胀和膜电位变化都受到损害。结论：因此，建议AQP7可能直接或间接在胞吐途径的远端部位起作用。

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