• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue
【24h】

Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue

机译：人骨髓和脂肪组织间充质干细胞的表征和表达分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human mesenchymal stem cells (MSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle and neuron. This differentiation potential makes MSC excellent candidates for cell-based tissue engineering. In this study, we have examined phenotypes and gene expression profile of the human adipose tissue-derived stromal cells (ATSC) in the undifferentiated states, and compared with that of bone marrow stromal cells (BMSC). ATSC were enzymatically released from adipose tissues from adult human donors and were expanded in monolayer with serial passages at confluence. BMSC were harvested from the metaphysis of adult human femur. Flowcytometric analysis showed that ATSC have a marker expression that is similar to that of BMSC. ATSC expressed CD29, CD44, CD90, CD105 and were absent for HLA-DR and c-kit expression. Under appropriate culture conditions, MSC were induced to differentiate to the osteoblast, adipocyte, and chondrogenic lineages. ATSC were superior to BMSC in respect to maintenance of proliferating ability, and microarray analysis of gene expression revealed differentially expressed genes between ATSC and BMSC. The proliferating ability and differentiation potential of ATSC were variable according to the culture condition. The similarities of the phenotypes and the gene expression profiles between ATSC and BMSC could have broad implications for human tissue engineering. Copyright (C) 2004 S. Karger AG, Basel.
机译：据报道，人类间充质干细胞（MSC）存在于骨髓，脂肪组织，真皮，肌肉和外周血中，具有沿不同谱系分化的潜力，包括形成骨骼，软骨，脂肪，肌肉和神经元的谱系。这种分化潜力使MSC成为基于细胞的组织工程的极佳候选者。在这项研究中，我们检查了未分化状态下人脂肪组织来源的基质细胞（ATSC）的表型和基因表达谱，并与骨髓基质细胞（BMSC）进行了比较。从成年人类供体的脂肪组织中酶促释放ATSC，并在汇合时通过连续传代以单层形式扩增。 BMSC从成人股骨干meta端收获。流式细胞仪分析表明，ATSC的标志物表达与BMSC相似。 ATSC表达CD29，CD44，CD90，CD105，而HLA-DR和c-kit表达不存在。在适当的培养条件下，诱导MSC分化为成骨细胞，脂肪细胞和成软骨细胞系。在维持增殖能力方面，ATSC优于BMSC，基因表达的微阵列分析揭示了ATSC和BMSC之间差异表达的基因。 ATSC的增殖能力和分化潜能根据培养条件而变化。 ATSC和BMSC之间的表型和基因表达谱的相似性可能对人体组织工程具有广泛的意义。版权所有（C）2004 S.Karger AG，巴塞尔。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号