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首页> 外文期刊>Cellular Physiology and Biochemistry >Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
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Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line

机译：促红细胞生成素在UT-7细胞系中对蛋白酪氨酸磷酸酶1B的调节

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Background/Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototypic member of the PTP family is PTP1B, a widely expressed non-receptor PTP located both in cytosol and intracellular membranes via its hydrophobic C-terminal targeting sequence. PTP1B has been implicated in the regulation of signaling pathways involving tyrosine phosphorylation induced by growth factors, cytokines, and hormones, such as the downregulation of erythropoietin and insulin receptors. However, little is known about which factor modulates the activity of this enzyme. Methods: The effect of Epo on PTP1B expression was studied in the UT-7 Epo-dependent cell line. PTP1B expression was analyzed under different conditions by Real-Time PCR and Western blot, while PTP1B phosphatase activity was determined by a p-nitrophenylphosphate hydrolysis assay. Results: Epo rapidly induced an increased expression of PTP1B which was associated with higher PTP1B tyrosine phosphorylation and phosphatase activity. The action of Epo on PTP1B induction involved Janus Kinase 2 (JAK2) and Phosphatidylinositol-3 kinase (PI3K). Conclusion: The results allow us to suggest for the first time that, besides modulating Epo/Epo receptor signaling, PTP1B undergoes feedback regulation by Epo. Copyright (c) 2007 S. Karger AG, Basel.
机译：背景/目的：由于酪氨酸残基的可逆磷酸化是促红细胞生成素（Epo）激活的细胞信号通路中的关键事件，因此注意力已集中在蛋白质酪氨酸磷酸酶（PTP）及其与蛋白质酪氨酸激酶的协同作用上。 PTP家族的原型成员是PTP1B，它是一种广泛表达的非受体PTP，通过其疏水性C端靶向序列位于细胞质和细胞内膜中。 PTP1B与涉及由生长因子，细胞因子和激素诱导的酪氨酸磷酸化的信号通路的调节有关，例如促红细胞生成素和胰岛素受体的下调。然而，对于哪个因子调节该酶的活性知之甚少。方法：在UT-7 Epo依赖性细胞系中研究了Epo对PTP1B表达的影响。通过实时PCR和Western印迹在不同条件下分析PTP1B的表达，而通过对硝基苯基磷酸酯水解法测定PTP1B磷酸酶的活性。结果：Epo迅速诱导PTP1B表达增加，这与较高的PTP1B酪氨酸磷酸化和磷酸酶活性有关。 Epo对PTP1B的诱导作用涉及Janus激酶2（JAK2）和Phosphatidylinositol-3激酶（PI3K）。结论：该结果使我们首次提出，除了调节Epo / Epo受体信号传导外，PTP1B还受到Epo的反馈调节。版权所有（c）2007 S.Karger AG，巴塞尔。

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