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首页> 外文期刊>Cellular Physiology and Biochemistry >Functional analyses of a N-terminal splice variant of the alpha subunit of the epithelial sodium channel
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Functional analyses of a N-terminal splice variant of the alpha subunit of the epithelial sodium channel

机译：上皮钠通道的α亚基的N末端剪接变体的功能分析

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摘要

The amiloride-sensitive epithelial sodium channel (ENaC) is the limiting step for sodium absorption in epithelial cells of the distal nephron, distal colon, airways and excretory ducts of several glands. In vivo and in vitro studies showed that the alpha subunit of ENaC is necessary for the expression of functional channels. Using RT-PCR strategy, a novel N-terminal splice variant has been identified which deletes 49 amino acids in the N-terminal region of the mouse alpha ENaC subunit. In oocytes expressing the alpha ENaC splice variant, together with beta and gamma ENaC subunits, amiloride-sensitive currents were less than 20% of values obtained with the wild type ENaC. The single channel conductance and the ionic selectivity were similar and there was only a minor decrease in the level of expression of the protein at the oocyte surface. These findings indicate that the deleted sequence in the N-terminal part of the mouse and rat alpha ENaC subunitmight play a role in the regulation of the activity of expressed ENaC channels. Copyright (C) 2001 S. Karger AG, Basel. [References: 23]
机译：阿米洛利敏感的上皮钠通道（ENaC）是肾的远端肾，远端结肠，气道和多个腺体分泌管上皮细胞中钠吸收的限制步骤。体内和体外研究表明，ENaC的α亚基是表达功能通道所必需的。使用RT-PCR策略，已鉴定出新颖的N末端剪接变体，其删除了小鼠αENaC亚基的N末端区域的49个氨基酸。在表达αENaC剪接变体以及β和γENaC亚基的卵母细胞中，阿米洛利敏感电流小于野生型ENaC获得的值的20％。单通道电导率和离子选择性相似，卵母细胞表面蛋白质表达水平仅略有下降。这些发现表明，在小鼠和大鼠的αENaC亚基的N末端部分中缺失的序列可能在表达的ENaC通道活性的调节中起作用。版权所有（C）2001 S.Karger AG，巴塞尔。 [参考：23]

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